Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse

Abstract In neonatal mice motoneurons excite Renshaw cells by releasing both acetylcholine (ACh) and glutamate. These two neurotransmitters activate two types of nicotinic receptors (nAChRs) (the homomeric α7 receptors and the heteromeric α*ß* receptors) as well as the two types of glutamate recept...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Boris Lamotte d’Incamps, Gardave S. Bhumbra, Joshua D. Foster, Marco Beato, Philippe Ascher
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
R
Q
Acceso en línea:https://doaj.org/article/298f339f82324a88a81b866d2df8df6f
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:298f339f82324a88a81b866d2df8df6f
record_format dspace
spelling oai:doaj.org-article:298f339f82324a88a81b866d2df8df6f2021-12-02T12:30:54ZSegregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse10.1038/s41598-017-04266-82045-2322https://doaj.org/article/298f339f82324a88a81b866d2df8df6f2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04266-8https://doaj.org/toc/2045-2322Abstract In neonatal mice motoneurons excite Renshaw cells by releasing both acetylcholine (ACh) and glutamate. These two neurotransmitters activate two types of nicotinic receptors (nAChRs) (the homomeric α7 receptors and the heteromeric α*ß* receptors) as well as the two types of glutamate receptors (GluRs) (AMPARs and NMDARs). Using paired recordings, we confirm that a single motoneuron can release both transmitters on a single post-synaptic Renshaw cell. We then show that co-transmission is preserved in adult animals. Kinetic analysis of miniature EPSCs revealed quantal release of mixed events associating AMPARs and NMDARs, as well as α7 and α*ß* nAChRs, but no evidence was found for mEPSCs associating nAChRs with GluRs. Bayesian Quantal Analysis (BQA) of evoked EPSCs showed that the number of functional contacts on a single Renshaw cell is more than halved when the nicotinic receptors are blocked, confirming that the two neurotransmitters systems are segregated. Our observations can be explained if ACh and glutamate are released from common vesicles onto spatially segregated post-synaptic receptors clusters, but a pre-synaptic segregation of cholinergic and glutamatergic release sites is also possible.Boris Lamotte d’IncampsGardave S. BhumbraJoshua D. FosterMarco BeatoPhilippe AscherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Boris Lamotte d’Incamps
Gardave S. Bhumbra
Joshua D. Foster
Marco Beato
Philippe Ascher
Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse
description Abstract In neonatal mice motoneurons excite Renshaw cells by releasing both acetylcholine (ACh) and glutamate. These two neurotransmitters activate two types of nicotinic receptors (nAChRs) (the homomeric α7 receptors and the heteromeric α*ß* receptors) as well as the two types of glutamate receptors (GluRs) (AMPARs and NMDARs). Using paired recordings, we confirm that a single motoneuron can release both transmitters on a single post-synaptic Renshaw cell. We then show that co-transmission is preserved in adult animals. Kinetic analysis of miniature EPSCs revealed quantal release of mixed events associating AMPARs and NMDARs, as well as α7 and α*ß* nAChRs, but no evidence was found for mEPSCs associating nAChRs with GluRs. Bayesian Quantal Analysis (BQA) of evoked EPSCs showed that the number of functional contacts on a single Renshaw cell is more than halved when the nicotinic receptors are blocked, confirming that the two neurotransmitters systems are segregated. Our observations can be explained if ACh and glutamate are released from common vesicles onto spatially segregated post-synaptic receptors clusters, but a pre-synaptic segregation of cholinergic and glutamatergic release sites is also possible.
format article
author Boris Lamotte d’Incamps
Gardave S. Bhumbra
Joshua D. Foster
Marco Beato
Philippe Ascher
author_facet Boris Lamotte d’Incamps
Gardave S. Bhumbra
Joshua D. Foster
Marco Beato
Philippe Ascher
author_sort Boris Lamotte d’Incamps
title Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse
title_short Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse
title_full Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse
title_fullStr Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse
title_full_unstemmed Segregation of glutamatergic and cholinergic transmission at the mixed motoneuron Renshaw cell synapse
title_sort segregation of glutamatergic and cholinergic transmission at the mixed motoneuron renshaw cell synapse
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/298f339f82324a88a81b866d2df8df6f
work_keys_str_mv AT borislamottedincamps segregationofglutamatergicandcholinergictransmissionatthemixedmotoneuronrenshawcellsynapse
AT gardavesbhumbra segregationofglutamatergicandcholinergictransmissionatthemixedmotoneuronrenshawcellsynapse
AT joshuadfoster segregationofglutamatergicandcholinergictransmissionatthemixedmotoneuronrenshawcellsynapse
AT marcobeato segregationofglutamatergicandcholinergictransmissionatthemixedmotoneuronrenshawcellsynapse
AT philippeascher segregationofglutamatergicandcholinergictransmissionatthemixedmotoneuronrenshawcellsynapse
_version_ 1718394266437287936