Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells

Abstract Early “T cell activation” events are initiated within the lipid microenvironment of the plasma membrane. Role of lipid membrane order (Lo) in spatiotemporal signaling through the antigen receptor in T cells is posited but remains unclear. We have examined the role of membrane order (Lo)/dis...

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Autores principales:	Soumini Sengupta, Ritesh Karsalia, Amanda Morrissey, Anil K. Bamezai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/29be056c74c94ae0b4f0c8490a3f5777
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spelling	oai:doaj.org-article:29be056c74c94ae0b4f0c8490a3f57772021-12-02T15:22:57ZCholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells10.1038/s41598-021-93403-52045-2322https://doaj.org/article/29be056c74c94ae0b4f0c8490a3f57772021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93403-5https://doaj.org/toc/2045-2322Abstract Early “T cell activation” events are initiated within the lipid microenvironment of the plasma membrane. Role of lipid membrane order (Lo) in spatiotemporal signaling through the antigen receptor in T cells is posited but remains unclear. We have examined the role of membrane order (Lo)/disorder (Ld) in antigen specific CD4+ T cell activation and clonal expansion by first creating membrane disorder, and then reconstituting membrane order by inserting cholesterol into the disordered plasma membrane. Significant revival of antigen specific CD4+ T cell proliferative response was observed after reconstituting the disrupted membrane order with cholesterol. These reconstitution experiments illustrate Koch’s postulate by demonstrating that cholesterol-dependent membrane order (Lo) is critical for responses generated by CD4+ T cells and point to the importance of membrane order and lipid microenvironment in signaling through T cell membrane antigen receptors.Soumini SenguptaRitesh KarsaliaAmanda MorrisseyAnil K. BamezaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Soumini Sengupta Ritesh Karsalia Amanda Morrissey Anil K. Bamezai Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells
description	Abstract Early “T cell activation” events are initiated within the lipid microenvironment of the plasma membrane. Role of lipid membrane order (Lo) in spatiotemporal signaling through the antigen receptor in T cells is posited but remains unclear. We have examined the role of membrane order (Lo)/disorder (Ld) in antigen specific CD4+ T cell activation and clonal expansion by first creating membrane disorder, and then reconstituting membrane order by inserting cholesterol into the disordered plasma membrane. Significant revival of antigen specific CD4+ T cell proliferative response was observed after reconstituting the disrupted membrane order with cholesterol. These reconstitution experiments illustrate Koch’s postulate by demonstrating that cholesterol-dependent membrane order (Lo) is critical for responses generated by CD4+ T cells and point to the importance of membrane order and lipid microenvironment in signaling through T cell membrane antigen receptors.
format	article
author	Soumini Sengupta Ritesh Karsalia Amanda Morrissey Anil K. Bamezai
author_facet	Soumini Sengupta Ritesh Karsalia Amanda Morrissey Anil K. Bamezai
author_sort	Soumini Sengupta
title	Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells
title_short	Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells
title_full	Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells
title_fullStr	Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells
title_full_unstemmed	Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells
title_sort	cholesterol-dependent plasma membrane order (lo) is critical for antigen-specific clonal expansion of cd4+ t cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/29be056c74c94ae0b4f0c8490a3f5777
work_keys_str_mv	AT souminisengupta cholesteroldependentplasmamembraneorderloiscriticalforantigenspecificclonalexpansionofcd4tcells AT riteshkarsalia cholesteroldependentplasmamembraneorderloiscriticalforantigenspecificclonalexpansionofcd4tcells AT amandamorrissey cholesteroldependentplasmamembraneorderloiscriticalforantigenspecificclonalexpansionofcd4tcells AT anilkbamezai cholesteroldependentplasmamembraneorderloiscriticalforantigenspecificclonalexpansionofcd4tcells
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Cholesterol-dependent plasma membrane order (Lo) is critical for antigen-specific clonal expansion of CD4+ T cells

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