Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines

Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines – one heterozygous (MRli003-A-1) and one ho...

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Autores principales:	Fangfang Zhang, Anna B. Meier, Karl-Ludwig Laugwitz, Tatjana Dorn, Alessandra Moretti
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2b4c75351f3e433ba604d486cc93cab9
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spelling	oai:doaj.org-article:2b4c75351f3e433ba604d486cc93cab92021-12-04T04:33:28ZGeneration of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines1873-506110.1016/j.scr.2021.102612https://doaj.org/article/2b4c75351f3e433ba604d486cc93cab92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1873506121004591https://doaj.org/toc/1873-5061Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines – one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) – by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential and a normal karyotype.Fangfang ZhangAnna B. MeierKarl-Ludwig LaugwitzTatjana DornAlessandra MorettiElsevierarticleBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102612- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Fangfang Zhang Anna B. Meier Karl-Ludwig Laugwitz Tatjana Dorn Alessandra Moretti Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines
description	Myosin-10, also known as non-muscle myosin IIB, is a cytoskeletal protein implicated in cardiac development and disease. In humans, it is encoded by the MYH10 gene. Using CRISPR/Cas9 gene editing technology, we generated two MYH10 knockout human iPSC lines – one heterozygous (MRli003-A-1) and one homozygous (MRli003-A-2) – by introducing a frameshift deletion in exon 2. We then verified that both lines had maintained pluripotency, parental cell morphology, trilineage differentiation potential and a normal karyotype.
format	article
author	Fangfang Zhang Anna B. Meier Karl-Ludwig Laugwitz Tatjana Dorn Alessandra Moretti
author_facet	Fangfang Zhang Anna B. Meier Karl-Ludwig Laugwitz Tatjana Dorn Alessandra Moretti
author_sort	Fangfang Zhang
title	Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines
title_short	Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines
title_full	Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines
title_fullStr	Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines
title_full_unstemmed	Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines
title_sort	generation of heterozygous (mrli003-a-1) and homozygous (mrli003-a-2) myh10 knockout human ipsc lines
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2b4c75351f3e433ba604d486cc93cab9
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Generation of heterozygous (MRli003-A-1) and homozygous (MRli003-A-2) MYH10 knockout human iPSC lines

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