A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.

Many bacterial species modify their DNA with the addition of sulfur to phosphate groups, a modification known as DNA phosphorothioation. DndA is known to act as a cysteine desulfurase, catalyzing a key biochemical step in phosphorothioation. However, bioinformatic analysis revealed that 19 out of th...

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Autores principales:	Xianhui An, Wei Xiong, Yan Yang, Fuhou Li, Xiufen Zhou, Zhijun Wang, Zixin Deng, Jingdan Liang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c38c0b952724b59820f5cc3f2169bb2
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spelling	oai:doaj.org-article:2c38c0b952724b59820f5cc3f2169bb22021-11-18T08:05:36ZA novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.1932-620310.1371/journal.pone.0051265https://doaj.org/article/2c38c0b952724b59820f5cc3f2169bb22012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23240007/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Many bacterial species modify their DNA with the addition of sulfur to phosphate groups, a modification known as DNA phosphorothioation. DndA is known to act as a cysteine desulfurase, catalyzing a key biochemical step in phosphorothioation. However, bioinformatic analysis revealed that 19 out of the 31 known dnd gene clusters, contain only four genes (dndB-E), lacking a key cysteine desulfurase corresponding gene. There are multiple cysteine desulfurase genes in Escherichia coli, but which one of them participates into DNA phosphorothioation is unknown. Here, by employing heterologous expression of the Salmonella enterica dnd gene cluster named dptBCDE in three E. coli mutants, each of which lacked a different cysteine desulfurase gene, we show that IscS is the only cysteine desulfurase that collaborates with dptB-E, resulting in DNA phosphorothioation. Using a bacterial two-hybrid system, protein interactions between IscS and DptC, and IscS and DptE were identified. Our findings revealed IscS as a key participant in DNA phosphorothioation and lay the basis for in-depth analysis of the DNA phosphorothioation biochemical pathway.Xianhui AnWei XiongYan YangFuhou LiXiufen ZhouZhijun WangZixin DengJingdan LiangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51265 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xianhui An Wei Xiong Yan Yang Fuhou Li Xiufen Zhou Zhijun Wang Zixin Deng Jingdan Liang A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.
description	Many bacterial species modify their DNA with the addition of sulfur to phosphate groups, a modification known as DNA phosphorothioation. DndA is known to act as a cysteine desulfurase, catalyzing a key biochemical step in phosphorothioation. However, bioinformatic analysis revealed that 19 out of the 31 known dnd gene clusters, contain only four genes (dndB-E), lacking a key cysteine desulfurase corresponding gene. There are multiple cysteine desulfurase genes in Escherichia coli, but which one of them participates into DNA phosphorothioation is unknown. Here, by employing heterologous expression of the Salmonella enterica dnd gene cluster named dptBCDE in three E. coli mutants, each of which lacked a different cysteine desulfurase gene, we show that IscS is the only cysteine desulfurase that collaborates with dptB-E, resulting in DNA phosphorothioation. Using a bacterial two-hybrid system, protein interactions between IscS and DptC, and IscS and DptE were identified. Our findings revealed IscS as a key participant in DNA phosphorothioation and lay the basis for in-depth analysis of the DNA phosphorothioation biochemical pathway.
format	article
author	Xianhui An Wei Xiong Yan Yang Fuhou Li Xiufen Zhou Zhijun Wang Zixin Deng Jingdan Liang
author_facet	Xianhui An Wei Xiong Yan Yang Fuhou Li Xiufen Zhou Zhijun Wang Zixin Deng Jingdan Liang
author_sort	Xianhui An
title	A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.
title_short	A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.
title_full	A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.
title_fullStr	A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.
title_full_unstemmed	A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.
title_sort	novel target of iscs in escherichia coli: participating in dna phosphorothioation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2c38c0b952724b59820f5cc3f2169bb2
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A novel target of IscS in Escherichia coli: participating in DNA phosphorothioation.

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