Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens

ABSTRACT The demand for a prophylactic vaccine against methicillin-resistant Staphylococcus aureus (MRSA) has motivated numerous dedicated research groups to design and develop such a vaccine. In this study, we have developed a multivalent vaccine, Sta-V5, composed of five conserved antigens involve...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jian Deng, Xiaolei Wang, Bao-Zhong Zhang, Peng Gao, Qiubin Lin, Richard Yi-Tsun Kao, Kenth Gustafsson, Kwok-Yung Yuen, Jian-Dong Huang
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	γδ T cell MRSA multivalent vaccine Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2c8355ae80914d0ebaedda23bd961a80
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2c8355ae80914d0ebaedda23bd961a80
record_format	dspace
spelling	oai:doaj.org-article:2c8355ae80914d0ebaedda23bd961a802021-11-15T15:27:32ZBroad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens10.1128/mSphere.00362-192379-5042https://doaj.org/article/2c8355ae80914d0ebaedda23bd961a802019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00362-19https://doaj.org/toc/2379-5042ABSTRACT The demand for a prophylactic vaccine against methicillin-resistant Staphylococcus aureus (MRSA) has motivated numerous dedicated research groups to design and develop such a vaccine. In this study, we have developed a multivalent vaccine, Sta-V5, composed of five conserved antigens involved in three important virulence mechanisms. This prototype vaccine conferred up to 100% protection against multiple epidemiologically relevant S. aureus isolates in five different murine disease models. The vaccine not only elicits functional antibodies that mediate opsonophagocytic killing of S. aureus but also mounts robust antigen-specific T-cell responses. In addition, our data implied that γδ T cells contribute to the protection induced by Sta-V5 in a murine skin infection model. IMPORTANCE Staphylococcus aureus infections, especially MRSA infections, are becoming a major global health issue and are resulting in mortality rates that are increasing every year. However, an effective vaccine is lacking due to the complexity of the infection process of S. aureus. In this study, we found that the addition of two novel protein components to three well-studied vaccine candidates significantly improved the efficacy of the combined vaccine. Furthermore, the five-component vaccine not only elicits a robust antibody response but also induces cytokine secretion by T cells, making it a promising vaccine candidate to fill the void.Jian DengXiaolei WangBao-Zhong ZhangPeng GaoQiubin LinRichard Yi-Tsun KaoKenth GustafssonKwok-Yung YuenJian-Dong HuangAmerican Society for Microbiologyarticleγδ T cellMRSAmultivalent vaccineMicrobiologyQR1-502ENmSphere, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	γδ T cell MRSA multivalent vaccine Microbiology QR1-502
spellingShingle	γδ T cell MRSA multivalent vaccine Microbiology QR1-502 Jian Deng Xiaolei Wang Bao-Zhong Zhang Peng Gao Qiubin Lin Richard Yi-Tsun Kao Kenth Gustafsson Kwok-Yung Yuen Jian-Dong Huang Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
description	ABSTRACT The demand for a prophylactic vaccine against methicillin-resistant Staphylococcus aureus (MRSA) has motivated numerous dedicated research groups to design and develop such a vaccine. In this study, we have developed a multivalent vaccine, Sta-V5, composed of five conserved antigens involved in three important virulence mechanisms. This prototype vaccine conferred up to 100% protection against multiple epidemiologically relevant S. aureus isolates in five different murine disease models. The vaccine not only elicits functional antibodies that mediate opsonophagocytic killing of S. aureus but also mounts robust antigen-specific T-cell responses. In addition, our data implied that γδ T cells contribute to the protection induced by Sta-V5 in a murine skin infection model. IMPORTANCE Staphylococcus aureus infections, especially MRSA infections, are becoming a major global health issue and are resulting in mortality rates that are increasing every year. However, an effective vaccine is lacking due to the complexity of the infection process of S. aureus. In this study, we found that the addition of two novel protein components to three well-studied vaccine candidates significantly improved the efficacy of the combined vaccine. Furthermore, the five-component vaccine not only elicits a robust antibody response but also induces cytokine secretion by T cells, making it a promising vaccine candidate to fill the void.
format	article
author	Jian Deng Xiaolei Wang Bao-Zhong Zhang Peng Gao Qiubin Lin Richard Yi-Tsun Kao Kenth Gustafsson Kwok-Yung Yuen Jian-Dong Huang
author_facet	Jian Deng Xiaolei Wang Bao-Zhong Zhang Peng Gao Qiubin Lin Richard Yi-Tsun Kao Kenth Gustafsson Kwok-Yung Yuen Jian-Dong Huang
author_sort	Jian Deng
title	Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
title_short	Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
title_full	Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
title_fullStr	Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
title_full_unstemmed	Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
title_sort	broad and effective protection against <named-content content-type="genus-species">staphylococcus aureus</named-content> is elicited by a multivalent vaccine formulated with novel antigens
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/2c8355ae80914d0ebaedda23bd961a80
work_keys_str_mv	AT jiandeng broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT xiaoleiwang broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT baozhongzhang broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT penggao broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT qiubinlin broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT richardyitsunkao broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT kenthgustafsson broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT kwokyungyuen broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens AT jiandonghuang broadandeffectiveprotectionagainstnamedcontentcontenttypegenusspeciesstaphylococcusaureusnamedcontentiselicitedbyamultivalentvaccineformulatedwithnovelantigens
_version_	1718427937463599104

Broad and Effective Protection against <named-content content-type="genus-species">Staphylococcus aureus</named-content> Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens

Ejemplares similares