Constrained G4 structures unveil topology specificity of known and new G4 binding proteins

Abstract G-quadruplexes (G4) are non-canonical secondary structures consisting in stacked tetrads of hydrogen-bonded guanines bases. An essential feature of G4 is their intrinsic polymorphic nature, which is characterized by the equilibrium between several conformations (also called topologies) and...

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Autores principales:	A. Pipier, A. Devaux, T. Lavergne, A. Adrait, Y. Couté, S. Britton, P. Calsou, J. F. Riou, E. Defrancq, D. Gomez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2c988d23c216412e911662ca68f6596a
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spelling	oai:doaj.org-article:2c988d23c216412e911662ca68f6596a2021-12-02T16:31:50ZConstrained G4 structures unveil topology specificity of known and new G4 binding proteins10.1038/s41598-021-92806-82045-2322https://doaj.org/article/2c988d23c216412e911662ca68f6596a2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92806-8https://doaj.org/toc/2045-2322Abstract G-quadruplexes (G4) are non-canonical secondary structures consisting in stacked tetrads of hydrogen-bonded guanines bases. An essential feature of G4 is their intrinsic polymorphic nature, which is characterized by the equilibrium between several conformations (also called topologies) and the presence of different types of loops with variable lengths. In cells, G4 functions rely on protein or enzymatic factors that recognize and promote or resolve these structures. In order to characterize new G4-dependent mechanisms, extensive researches aimed at identifying new G4 binding proteins. Using G-rich single-stranded oligonucleotides that adopt non-controlled G4 conformations, a large number of G4-binding proteins have been identified in vitro, but their specificity towards G4 topology remained unknown. Constrained G4 structures are biomolecular objects based on the use of a rigid cyclic peptide scaffold as a template for directing the intramolecular assembly of the anchored oligonucleotides into a single and stabilized G4 topology. Here, using various constrained RNA or DNA G4 as baits in human cell extracts, we establish the topology preference of several well-known G4-interacting factors. Moreover, we identify new G4-interacting proteins such as the NELF complex involved in the RNA-Pol II pausing mechanism, and we show that it impacts the clastogenic effect of the G4-ligand pyridostatin.A. PipierA. DevauxT. LavergneA. AdraitY. CoutéS. BrittonP. CalsouJ. F. RiouE. DefrancqD. GomezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Pipier A. Devaux T. Lavergne A. Adrait Y. Couté S. Britton P. Calsou J. F. Riou E. Defrancq D. Gomez Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
description	Abstract G-quadruplexes (G4) are non-canonical secondary structures consisting in stacked tetrads of hydrogen-bonded guanines bases. An essential feature of G4 is their intrinsic polymorphic nature, which is characterized by the equilibrium between several conformations (also called topologies) and the presence of different types of loops with variable lengths. In cells, G4 functions rely on protein or enzymatic factors that recognize and promote or resolve these structures. In order to characterize new G4-dependent mechanisms, extensive researches aimed at identifying new G4 binding proteins. Using G-rich single-stranded oligonucleotides that adopt non-controlled G4 conformations, a large number of G4-binding proteins have been identified in vitro, but their specificity towards G4 topology remained unknown. Constrained G4 structures are biomolecular objects based on the use of a rigid cyclic peptide scaffold as a template for directing the intramolecular assembly of the anchored oligonucleotides into a single and stabilized G4 topology. Here, using various constrained RNA or DNA G4 as baits in human cell extracts, we establish the topology preference of several well-known G4-interacting factors. Moreover, we identify new G4-interacting proteins such as the NELF complex involved in the RNA-Pol II pausing mechanism, and we show that it impacts the clastogenic effect of the G4-ligand pyridostatin.
format	article
author	A. Pipier A. Devaux T. Lavergne A. Adrait Y. Couté S. Britton P. Calsou J. F. Riou E. Defrancq D. Gomez
author_facet	A. Pipier A. Devaux T. Lavergne A. Adrait Y. Couté S. Britton P. Calsou J. F. Riou E. Defrancq D. Gomez
author_sort	A. Pipier
title	Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
title_short	Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
title_full	Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
title_fullStr	Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
title_full_unstemmed	Constrained G4 structures unveil topology specificity of known and new G4 binding proteins
title_sort	constrained g4 structures unveil topology specificity of known and new g4 binding proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2c988d23c216412e911662ca68f6596a
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Constrained G4 structures unveil topology specificity of known and new G4 binding proteins

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