Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells

The current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity.

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Autores principales:	Keith H. K. Wong, Shannon N. Tessier, David T. Miyamoto, Kathleen L. Miller, Lauren D. Bookstaver, Thomas R. Carey, Cleo J. Stannard, Vishal Thapar, Eric C. Tai, Kevin D. Vo, Erin S. Emmons, Haley M. Pleskow, Rebecca D. Sandlin, Lecia V. Sequist, David T. Ting, Daniel A. Haber, Shyamala Maheswaran, Shannon L. Stott, Mehmet Toner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2cb65508990f455f91b7d509541cd82f
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spelling	oai:doaj.org-article:2cb65508990f455f91b7d509541cd82f2021-12-02T14:40:33ZWhole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells10.1038/s41467-017-01705-y2041-1723https://doaj.org/article/2cb65508990f455f91b7d509541cd82f2017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01705-yhttps://doaj.org/toc/2041-1723The current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity.Keith H. K. WongShannon N. TessierDavid T. MiyamotoKathleen L. MillerLauren D. BookstaverThomas R. CareyCleo J. StannardVishal ThaparEric C. TaiKevin D. VoErin S. EmmonsHaley M. PleskowRebecca D. SandlinLecia V. SequistDavid T. TingDaniel A. HaberShyamala MaheswaranShannon L. StottMehmet TonerNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Keith H. K. Wong Shannon N. Tessier David T. Miyamoto Kathleen L. Miller Lauren D. Bookstaver Thomas R. Carey Cleo J. Stannard Vishal Thapar Eric C. Tai Kevin D. Vo Erin S. Emmons Haley M. Pleskow Rebecca D. Sandlin Lecia V. Sequist David T. Ting Daniel A. Haber Shyamala Maheswaran Shannon L. Stott Mehmet Toner Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
description	The current FDA-approved whole blood stabilization method for circulating tumor cell (CTC) isolation suffers from RNA degradation. Here the authors combine hypothermic preservation and antiplatelet strategies to stabilize whole blood up to 72 h without compromising CTC yield and RNA integrity.
format	article
author	Keith H. K. Wong Shannon N. Tessier David T. Miyamoto Kathleen L. Miller Lauren D. Bookstaver Thomas R. Carey Cleo J. Stannard Vishal Thapar Eric C. Tai Kevin D. Vo Erin S. Emmons Haley M. Pleskow Rebecca D. Sandlin Lecia V. Sequist David T. Ting Daniel A. Haber Shyamala Maheswaran Shannon L. Stott Mehmet Toner
author_facet	Keith H. K. Wong Shannon N. Tessier David T. Miyamoto Kathleen L. Miller Lauren D. Bookstaver Thomas R. Carey Cleo J. Stannard Vishal Thapar Eric C. Tai Kevin D. Vo Erin S. Emmons Haley M. Pleskow Rebecca D. Sandlin Lecia V. Sequist David T. Ting Daniel A. Haber Shyamala Maheswaran Shannon L. Stott Mehmet Toner
author_sort	Keith H. K. Wong
title	Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
title_short	Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
title_full	Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
title_fullStr	Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
title_full_unstemmed	Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
title_sort	whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2cb65508990f455f91b7d509541cd82f
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Whole blood stabilization for the microfluidic isolation and molecular characterization of circulating tumor cells

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