Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT

Persimmon (<i>Diospyros kaki</i> Thunb.) fruits are a remarkable source of carotenoids, which have shown protective effects against UV radiation in bacteria, fungi, algae, and plants. The aim of this study was to analyze the photoprotection provided by an acetone extract, rich in caroten...

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Autores principales:	Sara Gea-Botella, Bryan Moreno-Chamba, Laura de la Casa, Julio Salazar-Bermeo, Nuria Martí, María Concepción Martínez-Madrid, Manuel Valero, Domingo Saura
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Diospyros kaki</i> UV radiation photoprotection reactive oxygen species antimicrobial activity skin-care cosmetics Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/2cc6177a7c3d4d07ab8f2ed48822fcab
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spelling	oai:doaj.org-article:2cc6177a7c3d4d07ab8f2ed48822fcab2021-11-25T18:41:37ZCarotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT10.3390/pharmaceutics131118981999-4923https://doaj.org/article/2cc6177a7c3d4d07ab8f2ed48822fcab2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1898https://doaj.org/toc/1999-4923Persimmon (<i>Diospyros kaki</i> Thunb.) fruits are a remarkable source of carotenoids, which have shown protective effects against UV radiation in bacteria, fungi, algae, and plants. The aim of this study was to analyze the photoprotection provided by an acetone extract, rich in carotenoids and obtained from byproducts derived from the persimmon juice industry, against UV-induced cell death in the keratinocyte HaCaT cell line. For this purpose, the cytotoxicity and phototoxicity of carotenoid extract, as well as its intracellular reactive oxygen species (ROS) scavenging and anti-adhesive activities towards HaCaT cells, were evaluated. The in vitro permeation test provided information about the permeability of the carotenoid extract. Persimmon extracts, rich in carotenoids (PEC), were absorbed by HaCaT keratinocyte cells, which reduced the UV-induced intracellular ROS production in treated cells. Thus, PEC exerted a photoprotective and regenerative effect on UV-irradiated HaCaT cells, and this protection was UV dose-dependent. No cytotoxic effect was observed in HaCaT cultures at the concentration tested. PEC treatment also stimulated the adhesion capacity of skin microbiome to HaCaT cells, while exhibiting a significant anti-adhesive activity against all tested pathogens. In conclusion, PEC showed potential for use as a functional ingredient in skin-care products.Sara Gea-BotellaBryan Moreno-ChambaLaura de la CasaJulio Salazar-BermeoNuria MartíMaría Concepción Martínez-MadridManuel ValeroDomingo SauraMDPI AGarticle<i>Diospyros kaki</i>UV radiationphotoprotectionreactive oxygen speciesantimicrobial activityskin-care cosmeticsPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1898, p 1898 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Diospyros kaki</i> UV radiation photoprotection reactive oxygen species antimicrobial activity skin-care cosmetics Pharmacy and materia medica RS1-441
spellingShingle	<i>Diospyros kaki</i> UV radiation photoprotection reactive oxygen species antimicrobial activity skin-care cosmetics Pharmacy and materia medica RS1-441 Sara Gea-Botella Bryan Moreno-Chamba Laura de la Casa Julio Salazar-Bermeo Nuria Martí María Concepción Martínez-Madrid Manuel Valero Domingo Saura Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT
description	Persimmon (<i>Diospyros kaki</i> Thunb.) fruits are a remarkable source of carotenoids, which have shown protective effects against UV radiation in bacteria, fungi, algae, and plants. The aim of this study was to analyze the photoprotection provided by an acetone extract, rich in carotenoids and obtained from byproducts derived from the persimmon juice industry, against UV-induced cell death in the keratinocyte HaCaT cell line. For this purpose, the cytotoxicity and phototoxicity of carotenoid extract, as well as its intracellular reactive oxygen species (ROS) scavenging and anti-adhesive activities towards HaCaT cells, were evaluated. The in vitro permeation test provided information about the permeability of the carotenoid extract. Persimmon extracts, rich in carotenoids (PEC), were absorbed by HaCaT keratinocyte cells, which reduced the UV-induced intracellular ROS production in treated cells. Thus, PEC exerted a photoprotective and regenerative effect on UV-irradiated HaCaT cells, and this protection was UV dose-dependent. No cytotoxic effect was observed in HaCaT cultures at the concentration tested. PEC treatment also stimulated the adhesion capacity of skin microbiome to HaCaT cells, while exhibiting a significant anti-adhesive activity against all tested pathogens. In conclusion, PEC showed potential for use as a functional ingredient in skin-care products.
format	article
author	Sara Gea-Botella Bryan Moreno-Chamba Laura de la Casa Julio Salazar-Bermeo Nuria Martí María Concepción Martínez-Madrid Manuel Valero Domingo Saura
author_facet	Sara Gea-Botella Bryan Moreno-Chamba Laura de la Casa Julio Salazar-Bermeo Nuria Martí María Concepción Martínez-Madrid Manuel Valero Domingo Saura
author_sort	Sara Gea-Botella
title	Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT
title_short	Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT
title_full	Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT
title_fullStr	Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT
title_full_unstemmed	Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT
title_sort	carotenoids from persimmon (<i>diospyros kaki</i> thunb.) byproducts exert photoprotective, antioxidative and microbial anti-adhesive effects on hacat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2cc6177a7c3d4d07ab8f2ed48822fcab
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Carotenoids from Persimmon (<i>Diospyros kaki</i> Thunb.) Byproducts Exert Photoprotective, Antioxidative and Microbial Anti-Adhesive Effects on HaCaT

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