Post translational modifications of milk proteins in geographically diverse goat breeds

Abstract Goat milk is a source of nutrition in difficult areas and has lesser allerginicity than cow milk. It is leading in the area for nutraceutical formulation and drug development using goat mammary gland as a bioreactor. Post translational modifications of a protein regulate protein function, b...

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Autores principales:	P. K. Rout, M. Verma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2d20433b813747b2ab969eab8505c42a
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spelling	oai:doaj.org-article:2d20433b813747b2ab969eab8505c42a2021-12-02T13:19:29ZPost translational modifications of milk proteins in geographically diverse goat breeds10.1038/s41598-021-85094-92045-2322https://doaj.org/article/2d20433b813747b2ab969eab8505c42a2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85094-9https://doaj.org/toc/2045-2322Abstract Goat milk is a source of nutrition in difficult areas and has lesser allerginicity than cow milk. It is leading in the area for nutraceutical formulation and drug development using goat mammary gland as a bioreactor. Post translational modifications of a protein regulate protein function, biological activity, stabilization and interactions. The protein variants of goat milk from 10 breeds were studied for the post translational modifications by combining highly sensitive 2DE and Q-Exactive LC-MS/MS. Here we observed high levels of post translational modifications in 201 peptides of 120 goat milk proteins. The phosphosites observed for CSN2, CSN1S1, CSN1S2, CSN3 were 11P, 13P, 17P and 6P, respectively in 105 casein phosphopeptides. Whey proteins BLG and LALBA showed 19 and 4 phosphosites respectively. Post translational modification was observed in 45 low abundant non-casein milk proteins mainly associated with signal transduction, immune system, developmental biology and metabolism pathways. Pasp is reported for the first time in 47 sites. The rare conserved peptide sequence of (SSSEE) was observed in αS1 and αS2 casein. The functional roles of identified phosphopeptides included anti-microbial, DPP-IV inhibitory, anti-inflammatory and ACE inhibitory. This is first report from tropics, investigating post translational modifications in casein and non-casein goat milk proteins and studies their interactions.P. K. RoutM. VermaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q P. K. Rout M. Verma Post translational modifications of milk proteins in geographically diverse goat breeds
description	Abstract Goat milk is a source of nutrition in difficult areas and has lesser allerginicity than cow milk. It is leading in the area for nutraceutical formulation and drug development using goat mammary gland as a bioreactor. Post translational modifications of a protein regulate protein function, biological activity, stabilization and interactions. The protein variants of goat milk from 10 breeds were studied for the post translational modifications by combining highly sensitive 2DE and Q-Exactive LC-MS/MS. Here we observed high levels of post translational modifications in 201 peptides of 120 goat milk proteins. The phosphosites observed for CSN2, CSN1S1, CSN1S2, CSN3 were 11P, 13P, 17P and 6P, respectively in 105 casein phosphopeptides. Whey proteins BLG and LALBA showed 19 and 4 phosphosites respectively. Post translational modification was observed in 45 low abundant non-casein milk proteins mainly associated with signal transduction, immune system, developmental biology and metabolism pathways. Pasp is reported for the first time in 47 sites. The rare conserved peptide sequence of (SSSEE) was observed in αS1 and αS2 casein. The functional roles of identified phosphopeptides included anti-microbial, DPP-IV inhibitory, anti-inflammatory and ACE inhibitory. This is first report from tropics, investigating post translational modifications in casein and non-casein goat milk proteins and studies their interactions.
format	article
author	P. K. Rout M. Verma
author_facet	P. K. Rout M. Verma
author_sort	P. K. Rout
title	Post translational modifications of milk proteins in geographically diverse goat breeds
title_short	Post translational modifications of milk proteins in geographically diverse goat breeds
title_full	Post translational modifications of milk proteins in geographically diverse goat breeds
title_fullStr	Post translational modifications of milk proteins in geographically diverse goat breeds
title_full_unstemmed	Post translational modifications of milk proteins in geographically diverse goat breeds
title_sort	post translational modifications of milk proteins in geographically diverse goat breeds
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2d20433b813747b2ab969eab8505c42a
work_keys_str_mv	AT pkrout posttranslationalmodificationsofmilkproteinsingeographicallydiversegoatbreeds AT mverma posttranslationalmodificationsofmilkproteinsingeographicallydiversegoatbreeds
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Post translational modifications of milk proteins in geographically diverse goat breeds

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