A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses

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A versatile reverse genetics platform for SARS-CoV-2 and other positive-strand RNA viruses

Here the authors describe a simple reverse genetics method that relies on overlapping cDNA fragments for generation of positive-strand viruses including SARS-CoV-2 and characterize them in vitro and in vivo.

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Bibliographic Details
Main Authors:	Alberto A. Amarilla, Julian D. J. Sng, Rhys Parry, Joshua M. Deerain, James R. Potter, Yin Xiang Setoh, Daniel J. Rawle, Thuy T. Le, Naphak Modhiran, Xiaohui Wang, Nias Y. G. Peng, Francisco J. Torres, Alyssa Pyke, Jessica J. Harrison, Morgan E. Freney, Benjamin Liang, Christopher L. D. McMillan, Stacey T. M. Cheung, Darwin J. Da Costa Guevara, Joshua M. Hardy, Mark Bettington, David A. Muller, Fasséli Coulibaly, Frederick Moore, Roy A. Hall, Paul R. Young, Jason M. Mackenzie, Jody Hobson-Peters, Andreas Suhrbier, Daniel Watterson, Alexander A. Khromykh
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Science Q
Online Access:	https://doaj.org/article/2d42390e6b6242feac70593cf3d675b1
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Description
Summary:	Here the authors describe a simple reverse genetics method that relies on overlapping cDNA fragments for generation of positive-strand viruses including SARS-CoV-2 and characterize them in vitro and in vivo.