The Genetic Transformation of <named-content content-type="genus-species">Chlamydia pneumoniae</named-content>

The Genetic Transformation of <named-content content-type="genus-species">Chlamydia pneumoniae</named-content>

ABSTRACT We demonstrate the genetic transformation of Chlamydia pneumoniae using a plasmid shuttle vector system which generates stable transformants. The equine C. pneumoniae N16 isolate harbors the 7.5-kb plasmid pCpnE1. We constructed the plasmid vector pRSGFPCAT-Cpn containing a pCpnE1 backbone,...

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Auteurs principaux: Kensuke Shima, Maximilian Wanker, Rachel J. Skilton, Lesley T. Cutcliffe, Christiane Schnee, Thomas A. Kohl, Stefan Niemann, Javier Geijo, Matthias Klinger, Peter Timms, Thomas Rattei, Konrad Sachse, Ian N. Clarke, Jan Rupp
Format: article
Langue:EN
Publié: American Society for Microbiology 2018
Sujets:
Chlamydia felis
Chlamydia pneumoniae
genetic manipulation
plasmid shuttle vector
plasmid tropism
transformation
Microbiology
QR1-502
Accès en ligne:https://doaj.org/article/2d4f7be506794708bdf524dd71f61dc5
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https://doaj.org/article/2d4f7be506794708bdf524dd71f61dc5

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