The Genetic Transformation of <named-content content-type="genus-species">Chlamydia pneumoniae</named-content>

The Genetic Transformation of <named-content content-type="genus-species">Chlamydia pneumoniae</named-content>

ABSTRACT We demonstrate the genetic transformation of Chlamydia pneumoniae using a plasmid shuttle vector system which generates stable transformants. The equine C. pneumoniae N16 isolate harbors the 7.5-kb plasmid pCpnE1. We constructed the plasmid vector pRSGFPCAT-Cpn containing a pCpnE1 backbone,...

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Bibliographic Details
Main Authors: Kensuke Shima, Maximilian Wanker, Rachel J. Skilton, Lesley T. Cutcliffe, Christiane Schnee, Thomas A. Kohl, Stefan Niemann, Javier Geijo, Matthias Klinger, Peter Timms, Thomas Rattei, Konrad Sachse, Ian N. Clarke, Jan Rupp
Format: article
Language:EN
Published: American Society for Microbiology 2018
Subjects:
Chlamydia felis
Chlamydia pneumoniae
genetic manipulation
plasmid shuttle vector
plasmid tropism
transformation
Microbiology
QR1-502
Online Access:https://doaj.org/article/2d4f7be506794708bdf524dd71f61dc5
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