Hydroxyethyl cellulose matrix applied to serial crystallography

Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring c...

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Autores principales:	Michihiro Sugahara, Takanori Nakane, Tetsuya Masuda, Mamoru Suzuki, Shigeyuki Inoue, Changyong Song, Rie Tanaka, Toru Nakatsu, Eiichi Mizohata, Fumiaki Yumoto, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Keiji Numata, Eriko Nango, So Iwata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2f7a8196daea43459660ac4152b4bb81
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id	oai:doaj.org-article:2f7a8196daea43459660ac4152b4bb81
record_format	dspace
spelling	oai:doaj.org-article:2f7a8196daea43459660ac4152b4bb812021-12-02T15:06:27ZHydroxyethyl cellulose matrix applied to serial crystallography10.1038/s41598-017-00761-02045-2322https://doaj.org/article/2f7a8196daea43459660ac4152b4bb812017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00761-0https://doaj.org/toc/2045-2322Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.Michihiro SugaharaTakanori NakaneTetsuya MasudaMamoru SuzukiShigeyuki InoueChangyong SongRie TanakaToru NakatsuEiichi MizohataFumiaki YumotoKensuke TonoYasumasa JotiTakashi KameshimaTakaki HatsuiMakina YabashiOsamu NurekiKeiji NumataEriko NangoSo IwataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michihiro Sugahara Takanori Nakane Tetsuya Masuda Mamoru Suzuki Shigeyuki Inoue Changyong Song Rie Tanaka Toru Nakatsu Eiichi Mizohata Fumiaki Yumoto Kensuke Tono Yasumasa Joti Takashi Kameshima Takaki Hatsui Makina Yabashi Osamu Nureki Keiji Numata Eriko Nango So Iwata Hydroxyethyl cellulose matrix applied to serial crystallography
description	Serial femtosecond crystallography (SFX) allows structures of proteins to be determined at room temperature with minimal radiation damage. A highly viscous matrix acts as a crystal carrier for serial sample loading at a low flow rate that enables the determination of the structure, while requiring consumption of less than 1 mg of the sample. However, a reliable and versatile carrier matrix for a wide variety of protein samples is still elusive. Here we introduce a hydroxyethyl cellulose-matrix carrier, to determine the structure of three proteins. The de novo structure determination of proteinase K from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of the praseodymium atom was demonstrated using 3,000 diffraction images.
format	article
author	Michihiro Sugahara Takanori Nakane Tetsuya Masuda Mamoru Suzuki Shigeyuki Inoue Changyong Song Rie Tanaka Toru Nakatsu Eiichi Mizohata Fumiaki Yumoto Kensuke Tono Yasumasa Joti Takashi Kameshima Takaki Hatsui Makina Yabashi Osamu Nureki Keiji Numata Eriko Nango So Iwata
author_facet	Michihiro Sugahara Takanori Nakane Tetsuya Masuda Mamoru Suzuki Shigeyuki Inoue Changyong Song Rie Tanaka Toru Nakatsu Eiichi Mizohata Fumiaki Yumoto Kensuke Tono Yasumasa Joti Takashi Kameshima Takaki Hatsui Makina Yabashi Osamu Nureki Keiji Numata Eriko Nango So Iwata
author_sort	Michihiro Sugahara
title	Hydroxyethyl cellulose matrix applied to serial crystallography
title_short	Hydroxyethyl cellulose matrix applied to serial crystallography
title_full	Hydroxyethyl cellulose matrix applied to serial crystallography
title_fullStr	Hydroxyethyl cellulose matrix applied to serial crystallography
title_full_unstemmed	Hydroxyethyl cellulose matrix applied to serial crystallography
title_sort	hydroxyethyl cellulose matrix applied to serial crystallography
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2f7a8196daea43459660ac4152b4bb81
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Hydroxyethyl cellulose matrix applied to serial crystallography

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