Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion

Summary: It is still a challenge for synthesizing ‘cellular niche-mimics’ in vitro with satisfactory reproducibility and fidelity to recreate the natural niche components (e.g., extracellular matrices and soluble factors) for stem cell cultivation. Inspired by the massive amplification of hepatic pr...

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Autores principales:	Yuying Zhang, Anqi Guo, Cheng Lyu, Ran Bi, Zhaozhao Wu, Wenjing Li, Peng Zhao, Yudi Niu, Jie Na, Jianzhong Jeff Xi, Yanan Du
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Tissue engineering Biomimetics Stem cells research Science Q
Acceso en línea:	https://doaj.org/article/2fbacdcc82ff41d08de48209a19af3af
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spelling	oai:doaj.org-article:2fbacdcc82ff41d08de48209a19af3af2021-11-20T05:09:40ZSynthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion2589-004210.1016/j.isci.2021.103303https://doaj.org/article/2fbacdcc82ff41d08de48209a19af3af2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012724https://doaj.org/toc/2589-0042Summary: It is still a challenge for synthesizing ‘cellular niche-mimics’ in vitro with satisfactory reproducibility and fidelity to recreate the natural niche components (e.g., extracellular matrices and soluble factors) for stem cell cultivation. Inspired by the massive amplification of hepatic progenitor cells during liver fibrosis in vivo, here we optimized the in vitro liver fibrotic niches and subsequently harvested their bioactive ingredients as niche extracts (NEs). The fibrosis-relevant NE marginally outperformed Matrigel for phenotype maintenance of human embryonic stem cell (hESC)-derived hepatoblasts (HBs) and recapitulation of the pathological angiogenesis of hESC-derived endothelial cells both in 2D culture and 3D liver organoids. Finally, defined NE components (i.e., collagen III, IV, IL-17, IL-18 and M-CSF) were resolved by the quantitative proteomics which exhibited advantage over Matrigel for multi-passaged HB expansion. The pathology-relevant and tissue-specific NEs provide innovative and generalizable strategies for the discovery of optimal cellular niche and bioactive niche compositions.Yuying ZhangAnqi GuoCheng LyuRan BiZhaozhao WuWenjing LiPeng ZhaoYudi NiuJie NaJianzhong Jeff XiYanan DuElsevierarticleTissue engineeringBiomimeticsStem cells researchScienceQENiScience, Vol 24, Iss 11, Pp 103303- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tissue engineering Biomimetics Stem cells research Science Q
spellingShingle	Tissue engineering Biomimetics Stem cells research Science Q Yuying Zhang Anqi Guo Cheng Lyu Ran Bi Zhaozhao Wu Wenjing Li Peng Zhao Yudi Niu Jie Na Jianzhong Jeff Xi Yanan Du Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion
description	Summary: It is still a challenge for synthesizing ‘cellular niche-mimics’ in vitro with satisfactory reproducibility and fidelity to recreate the natural niche components (e.g., extracellular matrices and soluble factors) for stem cell cultivation. Inspired by the massive amplification of hepatic progenitor cells during liver fibrosis in vivo, here we optimized the in vitro liver fibrotic niches and subsequently harvested their bioactive ingredients as niche extracts (NEs). The fibrosis-relevant NE marginally outperformed Matrigel for phenotype maintenance of human embryonic stem cell (hESC)-derived hepatoblasts (HBs) and recapitulation of the pathological angiogenesis of hESC-derived endothelial cells both in 2D culture and 3D liver organoids. Finally, defined NE components (i.e., collagen III, IV, IL-17, IL-18 and M-CSF) were resolved by the quantitative proteomics which exhibited advantage over Matrigel for multi-passaged HB expansion. The pathology-relevant and tissue-specific NEs provide innovative and generalizable strategies for the discovery of optimal cellular niche and bioactive niche compositions.
format	article
author	Yuying Zhang Anqi Guo Cheng Lyu Ran Bi Zhaozhao Wu Wenjing Li Peng Zhao Yudi Niu Jie Na Jianzhong Jeff Xi Yanan Du
author_facet	Yuying Zhang Anqi Guo Cheng Lyu Ran Bi Zhaozhao Wu Wenjing Li Peng Zhao Yudi Niu Jie Na Jianzhong Jeff Xi Yanan Du
author_sort	Yuying Zhang
title	Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion
title_short	Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion
title_full	Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion
title_fullStr	Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion
title_full_unstemmed	Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion
title_sort	synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2fbacdcc82ff41d08de48209a19af3af
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Synthetic liver fibrotic niche extracts achieve in vitro hepatoblasts phenotype enhancement and expansion

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