Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens

Abstract Stem cells have been predicted to improve disease outcomes and patient lives. Steering stem cell fate - through controlling cell shape - may substantially accelerate progress towards this goal. As mesenchymal stromal cells (MSCs) are continuously exposed in vivo to a dynamically changing bi...

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Autores principales:	Brandan Walters, Tatiana Uynuk-Ool, Miriam Rothdiener, Julian Palm, Melanie L. Hart, Jan P. Stegemann, Bernd Rolauffs
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/300e8f51c90a4d3c98cf2320425ab844
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spelling	oai:doaj.org-article:300e8f51c90a4d3c98cf2320425ab8442021-12-02T12:31:51ZEngineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens10.1038/s41598-017-06794-92045-2322https://doaj.org/article/300e8f51c90a4d3c98cf2320425ab8442017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06794-9https://doaj.org/toc/2045-2322Abstract Stem cells have been predicted to improve disease outcomes and patient lives. Steering stem cell fate - through controlling cell shape - may substantially accelerate progress towards this goal. As mesenchymal stromal cells (MSCs) are continuously exposed in vivo to a dynamically changing biomechanical environment, we hypothesized that exogenous forces can be applied for engineering a variety of significantly different MSC shapes. We applied specific cyclic stretch regimens to human MSCs and quantitatively measured the resulting cell shape, alignment, and expression of smooth muscle (SMC) differentiation markers, as those have been associated with elongated morphology. As proof of principle, a range of different shapes, alignments, and correlating SMC marker levels were generated by varying strain, length, and repetition of stretch. However, the major determinant of biomechanically engineering cellular shape was the repetition of a chosen stretch regimen, indicating that the engineered shape and associated differentiation were complex non-linear processes relying on sustained biomechanical stimulation. Thus, forces are key regulators of stem cell shape and the targeted engineering of specific MSC shapes through biomechanical forces represents a novel mechanobiology concept that could exploit naturally occurring in vivo forces for improving stem cell fate in clinical regenerative therapies.Brandan WaltersTatiana Uynuk-OolMiriam RothdienerJulian PalmMelanie L. HartJan P. StegemannBernd RolauffsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Brandan Walters Tatiana Uynuk-Ool Miriam Rothdiener Julian Palm Melanie L. Hart Jan P. Stegemann Bernd Rolauffs Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
description	Abstract Stem cells have been predicted to improve disease outcomes and patient lives. Steering stem cell fate - through controlling cell shape - may substantially accelerate progress towards this goal. As mesenchymal stromal cells (MSCs) are continuously exposed in vivo to a dynamically changing biomechanical environment, we hypothesized that exogenous forces can be applied for engineering a variety of significantly different MSC shapes. We applied specific cyclic stretch regimens to human MSCs and quantitatively measured the resulting cell shape, alignment, and expression of smooth muscle (SMC) differentiation markers, as those have been associated with elongated morphology. As proof of principle, a range of different shapes, alignments, and correlating SMC marker levels were generated by varying strain, length, and repetition of stretch. However, the major determinant of biomechanically engineering cellular shape was the repetition of a chosen stretch regimen, indicating that the engineered shape and associated differentiation were complex non-linear processes relying on sustained biomechanical stimulation. Thus, forces are key regulators of stem cell shape and the targeted engineering of specific MSC shapes through biomechanical forces represents a novel mechanobiology concept that could exploit naturally occurring in vivo forces for improving stem cell fate in clinical regenerative therapies.
format	article
author	Brandan Walters Tatiana Uynuk-Ool Miriam Rothdiener Julian Palm Melanie L. Hart Jan P. Stegemann Bernd Rolauffs
author_facet	Brandan Walters Tatiana Uynuk-Ool Miriam Rothdiener Julian Palm Melanie L. Hart Jan P. Stegemann Bernd Rolauffs
author_sort	Brandan Walters
title	Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
title_short	Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
title_full	Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
title_fullStr	Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
title_full_unstemmed	Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
title_sort	engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/300e8f51c90a4d3c98cf2320425ab844
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Engineering the geometrical shape of mesenchymal stromal cells through defined cyclic stretch regimens

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