Efficient genome editing in filamentous fungi via an improved CRISPR‐Cas9 ribonucleoprotein method facilitated by chemical reagents
Summary DNA double‐strand break (DSB) repair induced by the RNA‐programmed nuclease Cas9 has become a popular method for genome editing. Direct genome editing via Cas9‐CRISPR gRNA (guide RNA) ribonucleoprotein (RNP) complexes assembled in vitro has also been successful in some fungi. However, the ef...
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| Main Authors: | , , , , , |
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| Format: | article |
| Language: | EN |
| Published: |
Wiley
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/309331d0df37402ba7234eca6db112d2 |
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