Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy

Abstract Performing long-term cell observations is a non-trivial task for conventional optical microscopy, since it is usually not compatible with environments of an incubator and its temperature and humidity requirements. Lensless holographic microscopy, being entirely based on semiconductor chips...

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Autores principales:	Agus Budi Dharmawan, Shinta Mariana, Gregor Scholz, Philipp Hörmann, Torben Schulze, Kuwat Triyana, Mayra Garcés-Schröder, Ingo Rustenbeck, Karsten Hiller, Hutomo Suryo Wasisto, Andreas Waag
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3154fd2bc4604154b7620892ee50020b
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spelling	oai:doaj.org-article:3154fd2bc4604154b7620892ee50020b2021-12-02T10:44:15ZNonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy10.1038/s41598-021-81098-72045-2322https://doaj.org/article/3154fd2bc4604154b7620892ee50020b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81098-7https://doaj.org/toc/2045-2322Abstract Performing long-term cell observations is a non-trivial task for conventional optical microscopy, since it is usually not compatible with environments of an incubator and its temperature and humidity requirements. Lensless holographic microscopy, being entirely based on semiconductor chips without lenses and without any moving parts, has proven to be a very interesting alternative to conventional microscopy. Here, we report on the integration of a computational parfocal feature, which operates based on wave propagation distribution analysis, to perform a fast autofocusing process. This unique non-mechanical focusing approach was implemented to keep the imaged object staying in-focus during continuous long-term and real-time recordings. A light-emitting diode (LED) combined with pinhole setup was used to realize a point light source, leading to a resolution down to 2.76 μm. Our approach delivers not only in-focus sharp images of dynamic cells, but also three-dimensional (3D) information on their (x, y, z)-positions. System reliability tests were conducted inside a sealed incubator to monitor cultures of three different biological living cells (i.e., MIN6, neuroblastoma (SH-SY5Y), and Prorocentrum minimum). Altogether, this autofocusing framework enables new opportunities for highly integrated microscopic imaging and dynamic tracking of moving objects in harsh environments with large sample areas.Agus Budi DharmawanShinta MarianaGregor ScholzPhilipp HörmannTorben SchulzeKuwat TriyanaMayra Garcés-SchröderIngo RustenbeckKarsten HillerHutomo Suryo WasistoAndreas WaagNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Agus Budi Dharmawan Shinta Mariana Gregor Scholz Philipp Hörmann Torben Schulze Kuwat Triyana Mayra Garcés-Schröder Ingo Rustenbeck Karsten Hiller Hutomo Suryo Wasisto Andreas Waag Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
description	Abstract Performing long-term cell observations is a non-trivial task for conventional optical microscopy, since it is usually not compatible with environments of an incubator and its temperature and humidity requirements. Lensless holographic microscopy, being entirely based on semiconductor chips without lenses and without any moving parts, has proven to be a very interesting alternative to conventional microscopy. Here, we report on the integration of a computational parfocal feature, which operates based on wave propagation distribution analysis, to perform a fast autofocusing process. This unique non-mechanical focusing approach was implemented to keep the imaged object staying in-focus during continuous long-term and real-time recordings. A light-emitting diode (LED) combined with pinhole setup was used to realize a point light source, leading to a resolution down to 2.76 μm. Our approach delivers not only in-focus sharp images of dynamic cells, but also three-dimensional (3D) information on their (x, y, z)-positions. System reliability tests were conducted inside a sealed incubator to monitor cultures of three different biological living cells (i.e., MIN6, neuroblastoma (SH-SY5Y), and Prorocentrum minimum). Altogether, this autofocusing framework enables new opportunities for highly integrated microscopic imaging and dynamic tracking of moving objects in harsh environments with large sample areas.
format	article
author	Agus Budi Dharmawan Shinta Mariana Gregor Scholz Philipp Hörmann Torben Schulze Kuwat Triyana Mayra Garcés-Schröder Ingo Rustenbeck Karsten Hiller Hutomo Suryo Wasisto Andreas Waag
author_facet	Agus Budi Dharmawan Shinta Mariana Gregor Scholz Philipp Hörmann Torben Schulze Kuwat Triyana Mayra Garcés-Schröder Ingo Rustenbeck Karsten Hiller Hutomo Suryo Wasisto Andreas Waag
author_sort	Agus Budi Dharmawan
title	Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
title_short	Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
title_full	Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
title_fullStr	Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
title_full_unstemmed	Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
title_sort	nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3154fd2bc4604154b7620892ee50020b
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Nonmechanical parfocal and autofocus features based on wave propagation distribution in lensfree holographic microscopy

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