The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis

A targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-drie...

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Autores principales:	Gloria I. Solano-Aguilar, Sukla Lakshman, Saebyeol Jang, Richi Gupta, Aleksey Molokin, Steven G. Schroeder, Patrick M. Gillevet, Joseph F. Urban
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	white button mushroom targeted metabolomics hippocampus cortex liver glycerophospholipids Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/31e71236449d420b9bce49c56830979b
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spelling	oai:doaj.org-article:31e71236449d420b9bce49c56830979b2021-11-25T18:20:52ZThe Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis10.3390/metabo111107792218-1989https://doaj.org/article/31e71236449d420b9bce49c56830979b2021-11-01T00:00:00Zhttps://www.mdpi.com/2218-1989/11/11/779https://doaj.org/toc/2218-1989A targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-dried WBM for six weeks. Tissue metabolomes were analyzed using targeted liquid chromatography-mass spectrometry (LC-MS) combined with chemical similarity enrichment analysis (ChemRICH) and correlated to WBM-induced changes in fecal microbiome composition. Results indicated that WBM can differentially modulate metabolites in liver, brain cortex and hippocampus of healthy pigs. Within the glycero-phospholipids, there was an increase in alkyl-acyl-phosphatidyl-cholines (PC-O 40:3) in the hippocampus of pigs fed six servings of WBM. A broader change in glycerophospholipids and sphingolipids was detected in the liver with a reduction in several lipid species in pigs fed both WBM diets but with an increase in amino acids known as precursors of neurotransmitters in the cortex of pigs fed six servings of WBM. Metabolomic changes were positively correlated with increased abundance of <i>Cryomorphaceae</i>, <i>Lachnospiraceae</i>, <i>Flammeovirgaceae</i> and <i>Ruminococcaceae</i> in the microbiome suggesting that WBM can also positively impact tissue metabolite composition.Gloria I. Solano-AguilarSukla LakshmanSaebyeol JangRichi GuptaAleksey MolokinSteven G. SchroederPatrick M. GillevetJoseph F. UrbanMDPI AGarticlewhite button mushroomtargeted metabolomicshippocampuscortexliverglycerophospholipidsMicrobiologyQR1-502ENMetabolites, Vol 11, Iss 779, p 779 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	white button mushroom targeted metabolomics hippocampus cortex liver glycerophospholipids Microbiology QR1-502
spellingShingle	white button mushroom targeted metabolomics hippocampus cortex liver glycerophospholipids Microbiology QR1-502 Gloria I. Solano-Aguilar Sukla Lakshman Saebyeol Jang Richi Gupta Aleksey Molokin Steven G. Schroeder Patrick M. Gillevet Joseph F. Urban The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis
description	A targeted metabolomic analysis was performed on tissues derived from pigs fed diets supplemented with white button mushrooms (WBM) to determine the effect on the liver and brain metabolome. Thirty-one pigs were fed a grower diet alone or supplemented with either three or six servings of freeze-dried WBM for six weeks. Tissue metabolomes were analyzed using targeted liquid chromatography-mass spectrometry (LC-MS) combined with chemical similarity enrichment analysis (ChemRICH) and correlated to WBM-induced changes in fecal microbiome composition. Results indicated that WBM can differentially modulate metabolites in liver, brain cortex and hippocampus of healthy pigs. Within the glycero-phospholipids, there was an increase in alkyl-acyl-phosphatidyl-cholines (PC-O 40:3) in the hippocampus of pigs fed six servings of WBM. A broader change in glycerophospholipids and sphingolipids was detected in the liver with a reduction in several lipid species in pigs fed both WBM diets but with an increase in amino acids known as precursors of neurotransmitters in the cortex of pigs fed six servings of WBM. Metabolomic changes were positively correlated with increased abundance of <i>Cryomorphaceae</i>, <i>Lachnospiraceae</i>, <i>Flammeovirgaceae</i> and <i>Ruminococcaceae</i> in the microbiome suggesting that WBM can also positively impact tissue metabolite composition.
format	article
author	Gloria I. Solano-Aguilar Sukla Lakshman Saebyeol Jang Richi Gupta Aleksey Molokin Steven G. Schroeder Patrick M. Gillevet Joseph F. Urban
author_facet	Gloria I. Solano-Aguilar Sukla Lakshman Saebyeol Jang Richi Gupta Aleksey Molokin Steven G. Schroeder Patrick M. Gillevet Joseph F. Urban
author_sort	Gloria I. Solano-Aguilar
title	The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis
title_short	The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis
title_full	The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis
title_fullStr	The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis
title_full_unstemmed	The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis
title_sort	effects of consuming white button mushroom <i>agaricus bisporus</i> on the brain and liver metabolome using a targeted metabolomic analysis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/31e71236449d420b9bce49c56830979b
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The Effects of Consuming White Button Mushroom <i>Agaricus bisporus</i> on the Brain and Liver Metabolome Using a Targeted Metabolomic Analysis

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