Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
Abstract Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis...
Guardado en:
Autores principales: | , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2021
|
Materias: | |
Acceso en línea: | https://doaj.org/article/321f058fc13c40f2a5009e1a5818fb5d |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:321f058fc13c40f2a5009e1a5818fb5d |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:321f058fc13c40f2a5009e1a5818fb5d2021-12-02T14:01:34ZLactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria10.1038/s41598-020-80921-x2045-2322https://doaj.org/article/321f058fc13c40f2a5009e1a5818fb5d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80921-xhttps://doaj.org/toc/2045-2322Abstract Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease.Kyung Mi YangJi-Sun KimHye-Sung KimYoung-Youn KimJeong-Kyu OhHye-Won JungDoo-Sang ParkKwang-Hak BaeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Kyung Mi Yang Ji-Sun Kim Hye-Sung Kim Young-Youn Kim Jeong-Kyu Oh Hye-Won Jung Doo-Sang Park Kwang-Hak Bae Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria |
description |
Abstract Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease. |
format |
article |
author |
Kyung Mi Yang Ji-Sun Kim Hye-Sung Kim Young-Youn Kim Jeong-Kyu Oh Hye-Won Jung Doo-Sang Park Kwang-Hak Bae |
author_facet |
Kyung Mi Yang Ji-Sun Kim Hye-Sung Kim Young-Youn Kim Jeong-Kyu Oh Hye-Won Jung Doo-Sang Park Kwang-Hak Bae |
author_sort |
Kyung Mi Yang |
title |
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria |
title_short |
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria |
title_full |
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria |
title_fullStr |
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria |
title_full_unstemmed |
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria |
title_sort |
lactobacillus reuteri an417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/321f058fc13c40f2a5009e1a5818fb5d |
work_keys_str_mv |
AT kyungmiyang lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria AT jisunkim lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria AT hyesungkim lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria AT youngyounkim lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria AT jeongkyuoh lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria AT hyewonjung lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria AT doosangpark lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria AT kwanghakbae lactobacillusreuterian417cellfreeculturesupernatantasanovelantibacterialagenttargetingoralpathogenicbacteria |
_version_ |
1718392131276505088 |