Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria

Abstract Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis...

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Autores principales: Kyung Mi Yang, Ji-Sun Kim, Hye-Sung Kim, Young-Youn Kim, Jeong-Kyu Oh, Hye-Won Jung, Doo-Sang Park, Kwang-Hak Bae
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Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/321f058fc13c40f2a5009e1a5818fb5d
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spelling oai:doaj.org-article:321f058fc13c40f2a5009e1a5818fb5d2021-12-02T14:01:34ZLactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria10.1038/s41598-020-80921-x2045-2322https://doaj.org/article/321f058fc13c40f2a5009e1a5818fb5d2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80921-xhttps://doaj.org/toc/2045-2322Abstract Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease.Kyung Mi YangJi-Sun KimHye-Sung KimYoung-Youn KimJeong-Kyu OhHye-Won JungDoo-Sang ParkKwang-Hak BaeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Kyung Mi Yang
Ji-Sun Kim
Hye-Sung Kim
Young-Youn Kim
Jeong-Kyu Oh
Hye-Won Jung
Doo-Sang Park
Kwang-Hak Bae
Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
description Abstract Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease.
format article
author Kyung Mi Yang
Ji-Sun Kim
Hye-Sung Kim
Young-Youn Kim
Jeong-Kyu Oh
Hye-Won Jung
Doo-Sang Park
Kwang-Hak Bae
author_facet Kyung Mi Yang
Ji-Sun Kim
Hye-Sung Kim
Young-Youn Kim
Jeong-Kyu Oh
Hye-Won Jung
Doo-Sang Park
Kwang-Hak Bae
author_sort Kyung Mi Yang
title Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
title_short Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
title_full Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
title_fullStr Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
title_full_unstemmed Lactobacillus reuteri AN417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
title_sort lactobacillus reuteri an417 cell-free culture supernatant as a novel antibacterial agent targeting oral pathogenic bacteria
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/321f058fc13c40f2a5009e1a5818fb5d
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