Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

CRISPR-Cas9 has enabled an unprecedented ability to manipulate the genome yet it is still poorly understood how target recognition functions at a molecular level. Here the authors use single-molecule FRET to probe Cas9-target interaction and identify distinct search and proofreading states.

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Auteurs principaux: Digvijay Singh, Samuel H. Sternberg, Jingyi Fei, Jennifer A. Doudna, Taekjip Ha
Format: article
Langue:EN
Publié: Nature Portfolio 2016
Sujets:
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Accès en ligne:https://doaj.org/article/32ed29bb68544d38a4398de244550193
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