Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

CRISPR-Cas9 has enabled an unprecedented ability to manipulate the genome yet it is still poorly understood how target recognition functions at a molecular level. Here the authors use single-molecule FRET to probe Cas9-target interaction and identify distinct search and proofreading states.

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Autores principales:	Digvijay Singh, Samuel H. Sternberg, Jingyi Fei, Jennifer A. Doudna, Taekjip Ha
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/32ed29bb68544d38a4398de244550193
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spelling	oai:doaj.org-article:32ed29bb68544d38a4398de2445501932021-12-02T17:31:50ZReal-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas910.1038/ncomms127782041-1723https://doaj.org/article/32ed29bb68544d38a4398de2445501932016-09-01T00:00:00Zhttps://doi.org/10.1038/ncomms12778https://doaj.org/toc/2041-1723CRISPR-Cas9 has enabled an unprecedented ability to manipulate the genome yet it is still poorly understood how target recognition functions at a molecular level. Here the authors use single-molecule FRET to probe Cas9-target interaction and identify distinct search and proofreading states.Digvijay SinghSamuel H. SternbergJingyi FeiJennifer A. DoudnaTaekjip HaNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Digvijay Singh Samuel H. Sternberg Jingyi Fei Jennifer A. Doudna Taekjip Ha Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
description	CRISPR-Cas9 has enabled an unprecedented ability to manipulate the genome yet it is still poorly understood how target recognition functions at a molecular level. Here the authors use single-molecule FRET to probe Cas9-target interaction and identify distinct search and proofreading states.
format	article
author	Digvijay Singh Samuel H. Sternberg Jingyi Fei Jennifer A. Doudna Taekjip Ha
author_facet	Digvijay Singh Samuel H. Sternberg Jingyi Fei Jennifer A. Doudna Taekjip Ha
author_sort	Digvijay Singh
title	Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
title_short	Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
title_full	Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
title_fullStr	Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
title_full_unstemmed	Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
title_sort	real-time observation of dna recognition and rejection by the rna-guided endonuclease cas9
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/32ed29bb68544d38a4398de244550193
work_keys_str_mv	AT digvijaysingh realtimeobservationofdnarecognitionandrejectionbythernaguidedendonucleasecas9 AT samuelhsternberg realtimeobservationofdnarecognitionandrejectionbythernaguidedendonucleasecas9 AT jingyifei realtimeobservationofdnarecognitionandrejectionbythernaguidedendonucleasecas9 AT jenniferadoudna realtimeobservationofdnarecognitionandrejectionbythernaguidedendonucleasecas9 AT taekjipha realtimeobservationofdnarecognitionandrejectionbythernaguidedendonucleasecas9
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Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9

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