Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity

The link between gRNA sequence and Cas9 activity is well established but the mechanism underlying this relationship is not well understood. Here the authors show that gRNA sequence primarily influences activity by dictating the time it takes for Cas9 to find the target site in a species-specific man...

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Autores principales:	E. A. Moreb, M. D. Lynch
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3315b484d90740b68962e62b99ef8601
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spelling	oai:doaj.org-article:3315b484d90740b68962e62b99ef86012021-12-02T18:00:10ZGenome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity10.1038/s41467-021-25339-32041-1723https://doaj.org/article/3315b484d90740b68962e62b99ef86012021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25339-3https://doaj.org/toc/2041-1723The link between gRNA sequence and Cas9 activity is well established but the mechanism underlying this relationship is not well understood. Here the authors show that gRNA sequence primarily influences activity by dictating the time it takes for Cas9 to find the target site in a species-specific manner.E. A. MorebM. D. LynchNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q E. A. Moreb M. D. Lynch Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
description	The link between gRNA sequence and Cas9 activity is well established but the mechanism underlying this relationship is not well understood. Here the authors show that gRNA sequence primarily influences activity by dictating the time it takes for Cas9 to find the target site in a species-specific manner.
format	article
author	E. A. Moreb M. D. Lynch
author_facet	E. A. Moreb M. D. Lynch
author_sort	E. A. Moreb
title	Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_short	Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_full	Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_fullStr	Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_full_unstemmed	Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_sort	genome dependent cas9/grna search time underlies sequence dependent grna activity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3315b484d90740b68962e62b99ef8601
work_keys_str_mv	AT eamoreb genomedependentcas9grnasearchtimeunderliessequencedependentgrnaactivity AT mdlynch genomedependentcas9grnasearchtimeunderliessequencedependentgrnaactivity
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Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity

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