Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity

Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity

The link between gRNA sequence and Cas9 activity is well established but the mechanism underlying this relationship is not well understood. Here the authors show that gRNA sequence primarily influences activity by dictating the time it takes for Cas9 to find the target site in a species-specific man...

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Auteurs principaux: E. A. Moreb, M. D. Lynch
Format: article
Langue:EN
Publié: Nature Portfolio 2021
Sujets:
Science
Q
Accès en ligne:https://doaj.org/article/3315b484d90740b68962e62b99ef8601
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spelling oai:doaj.org-article:3315b484d90740b68962e62b99ef86012021-12-02T18:00:10ZGenome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity10.1038/s41467-021-25339-32041-1723https://doaj.org/article/3315b484d90740b68962e62b99ef86012021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25339-3https://doaj.org/toc/2041-1723The link between gRNA sequence and Cas9 activity is well established but the mechanism underlying this relationship is not well understood. Here the authors show that gRNA sequence primarily influences activity by dictating the time it takes for Cas9 to find the target site in a species-specific manner.E. A. MorebM. D. LynchNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
E. A. Moreb
M. D. Lynch
Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
description The link between gRNA sequence and Cas9 activity is well established but the mechanism underlying this relationship is not well understood. Here the authors show that gRNA sequence primarily influences activity by dictating the time it takes for Cas9 to find the target site in a species-specific manner.
format article
author E. A. Moreb
M. D. Lynch
author_facet E. A. Moreb
M. D. Lynch
author_sort E. A. Moreb
title Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_short Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_full Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_fullStr Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_full_unstemmed Genome dependent Cas9/gRNA search time underlies sequence dependent gRNA activity
title_sort genome dependent cas9/grna search time underlies sequence dependent grna activity
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/3315b484d90740b68962e62b99ef8601
work_keys_str_mv AT eamoreb genomedependentcas9grnasearchtimeunderliessequencedependentgrnaactivity
AT mdlynch genomedependentcas9grnasearchtimeunderliessequencedependentgrnaactivity
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