Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?

Context: Rhodiola rosea L. (Crassulaceae) is well-known to contain flavonoids such as the herbacetin derivative rhodiosin. However, flavonoids are not typically used in quality control. Objective: This study analyses two flavonoids of R. rosea rhizomes and roots for their potential as analytical mar...

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Autores principales:	Zoltán Péter Zomborszki, Norbert Kúsz, Dezső Csupor, Wieland Peschel
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	hplc flavonoid Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/338d1bff53554f7c973a7936ba8eeb7c
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spelling	oai:doaj.org-article:338d1bff53554f7c973a7936ba8eeb7c2021-11-17T14:21:55ZRhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?1388-02091744-511610.1080/13880209.2019.1577460https://doaj.org/article/338d1bff53554f7c973a7936ba8eeb7c2019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1577460https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: Rhodiola rosea L. (Crassulaceae) is well-known to contain flavonoids such as the herbacetin derivative rhodiosin. However, flavonoids are not typically used in quality control. Objective: This study analyses two flavonoids of R. rosea rhizomes and roots for their potential as analytical markers. Materials and methods: Two constituents were isolated from ethanolic extracts via HPLC, identified via NMR and quantified via RP-HPLC. Presence and content variation was investigated according to extraction (solvent and repetitions), drying (temperature and duration) and sample origin (homogenously cultivated plants of different provenance, commercial samples). Results: Rhodiosin was identified as a main flavonoid, accompanied by 10-fold lower concentrated herbacetin. Both compounds were best extracted with 70–90% ethanol, but were also detectable in more aqueous extracts. Different drying conditions had no effect on the flavonoid content. These two flavonoids were consistently identified in rhizome and root extracts of over 100 R. rosea samples. Rhizomes tend to contain less flavonoids, with average ratios of rosavins to flavonoids of 1.4 (rhizomes) and 0.4 (roots). Provenance differences were detected in the range (rhodiosin plus herbacetin) of 760–6300 µg/mL extract corresponding to a maximum of approximately 0.5–4.2% (w/w) in the dry drug. Conclusions: For the first time, two main flavonoids present in R. rosea were quantified systematically. Rhodiosin and herbacetin can be detected simultaneously to phenylpropenoids or salidroside in authentic samples, influenced by the plant part examined and the plant origin. Rhodiosin and herbacetin may serve as additional marker to guarantee a consistent content of R. rosea products.Zoltán Péter ZomborszkiNorbert KúszDezső CsuporWieland PeschelTaylor & Francis GrouparticlehplcflavonoidTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 295-305 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	hplc flavonoid Therapeutics. Pharmacology RM1-950
spellingShingle	hplc flavonoid Therapeutics. Pharmacology RM1-950 Zoltán Péter Zomborszki Norbert Kúsz Dezső Csupor Wieland Peschel Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?
description	Context: Rhodiola rosea L. (Crassulaceae) is well-known to contain flavonoids such as the herbacetin derivative rhodiosin. However, flavonoids are not typically used in quality control. Objective: This study analyses two flavonoids of R. rosea rhizomes and roots for their potential as analytical markers. Materials and methods: Two constituents were isolated from ethanolic extracts via HPLC, identified via NMR and quantified via RP-HPLC. Presence and content variation was investigated according to extraction (solvent and repetitions), drying (temperature and duration) and sample origin (homogenously cultivated plants of different provenance, commercial samples). Results: Rhodiosin was identified as a main flavonoid, accompanied by 10-fold lower concentrated herbacetin. Both compounds were best extracted with 70–90% ethanol, but were also detectable in more aqueous extracts. Different drying conditions had no effect on the flavonoid content. These two flavonoids were consistently identified in rhizome and root extracts of over 100 R. rosea samples. Rhizomes tend to contain less flavonoids, with average ratios of rosavins to flavonoids of 1.4 (rhizomes) and 0.4 (roots). Provenance differences were detected in the range (rhodiosin plus herbacetin) of 760–6300 µg/mL extract corresponding to a maximum of approximately 0.5–4.2% (w/w) in the dry drug. Conclusions: For the first time, two main flavonoids present in R. rosea were quantified systematically. Rhodiosin and herbacetin can be detected simultaneously to phenylpropenoids or salidroside in authentic samples, influenced by the plant part examined and the plant origin. Rhodiosin and herbacetin may serve as additional marker to guarantee a consistent content of R. rosea products.
format	article
author	Zoltán Péter Zomborszki Norbert Kúsz Dezső Csupor Wieland Peschel
author_facet	Zoltán Péter Zomborszki Norbert Kúsz Dezső Csupor Wieland Peschel
author_sort	Zoltán Péter Zomborszki
title	Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?
title_short	Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?
title_full	Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?
title_fullStr	Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?
title_full_unstemmed	Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?
title_sort	rhodiosin and herbacetin in rhodiola rosea preparations: additional markers for quality control?
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/338d1bff53554f7c973a7936ba8eeb7c
work_keys_str_mv	AT zoltanpeterzomborszki rhodiosinandherbacetininrhodiolaroseapreparationsadditionalmarkersforqualitycontrol AT norbertkusz rhodiosinandherbacetininrhodiolaroseapreparationsadditionalmarkersforqualitycontrol AT dezsocsupor rhodiosinandherbacetininrhodiolaroseapreparationsadditionalmarkersforqualitycontrol AT wielandpeschel rhodiosinandherbacetininrhodiolaroseapreparationsadditionalmarkersforqualitycontrol
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Rhodiosin and herbacetin in Rhodiola rosea preparations: additional markers for quality control?

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