Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
Cryopreservation by droplet vitrification was applied to hazelnut (<i>Corylus avellana</i> L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i>in vitro</i> growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/350b3eb3b04248bba32a900662003198 |
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| Sumario: | Cryopreservation by droplet vitrification was applied to hazelnut (<i>Corylus avellana</i> L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i>in vitro</i> growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots. |
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