Cryopreservation of Hazelnut (Corylus avellana L.) Axillary Buds from In Vitro Shoots Using the Droplet Vitrification Method

Cryopreservation by droplet vitrification was applied to hazelnut (Corylus avellana L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from in vitro growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min...

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Autores principales:	Alessandra Sgueglia, Andrea Frattarelli, Adele Gentile, Gaia Urbinati, Simona Lucioli, Maria Antonietta Germanà, Emilia Caboni
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>in vitro</i> culture length of dehydration PVS2 PVS3 regrowth rooting Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/350b3eb3b04248bba32a900662003198
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spelling	oai:doaj.org-article:350b3eb3b04248bba32a9006620031982021-11-25T17:47:38ZCryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method10.3390/horticulturae71104942311-7524https://doaj.org/article/350b3eb3b04248bba32a9006620031982021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/494https://doaj.org/toc/2311-7524Cryopreservation by droplet vitrification was applied to hazelnut (<i>Corylus avellana</i> L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i>in vitro</i> growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots.Alessandra SguegliaAndrea FrattarelliAdele GentileGaia UrbinatiSimona LucioliMaria Antonietta GermanàEmilia CaboniMDPI AGarticle<i>in vitro</i> culturelength of dehydrationPVS2PVS3regrowthrootingPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 494, p 494 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>in vitro</i> culture length of dehydration PVS2 PVS3 regrowth rooting Plant culture SB1-1110
spellingShingle	<i>in vitro</i> culture length of dehydration PVS2 PVS3 regrowth rooting Plant culture SB1-1110 Alessandra Sgueglia Andrea Frattarelli Adele Gentile Gaia Urbinati Simona Lucioli Maria Antonietta Germanà Emilia Caboni Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
description	Cryopreservation by droplet vitrification was applied to hazelnut (<i>Corylus avellana</i> L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i>in vitro</i> growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots.
format	article
author	Alessandra Sgueglia Andrea Frattarelli Adele Gentile Gaia Urbinati Simona Lucioli Maria Antonietta Germanà Emilia Caboni
author_facet	Alessandra Sgueglia Andrea Frattarelli Adele Gentile Gaia Urbinati Simona Lucioli Maria Antonietta Germanà Emilia Caboni
author_sort	Alessandra Sgueglia
title	Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
title_short	Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
title_full	Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
title_fullStr	Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
title_full_unstemmed	Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method
title_sort	cryopreservation of hazelnut (<i>corylus avellana</i> l.) axillary buds from <i>in vitro</i> shoots using the droplet vitrification method
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/350b3eb3b04248bba32a900662003198
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Cryopreservation of Hazelnut (<i>Corylus avellana</i> L.) Axillary Buds from <i>In Vitro</i> Shoots Using the Droplet Vitrification Method

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