Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.

There is strong evidence from human and animal models that exposure to maternal hyperglycemia during in utero development can detrimentally affect fetal kidney development. Notwithstanding this knowledge, the precise effects of diabetic pregnancy on the key processes of kidney development are unclea...

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Autores principales:	Stacey N Hokke, James A Armitage, Victor G Puelles, Kieran M Short, Lynelle Jones, Ian M Smyth, John F Bertram, Luise A Cullen-McEwen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/354f71efdc2e42c79410921dbb514e3b
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spelling	oai:doaj.org-article:354f71efdc2e42c79410921dbb514e3b2021-11-18T07:53:41ZAltered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.1932-620310.1371/journal.pone.0058243https://doaj.org/article/354f71efdc2e42c79410921dbb514e3b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23516451/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203There is strong evidence from human and animal models that exposure to maternal hyperglycemia during in utero development can detrimentally affect fetal kidney development. Notwithstanding this knowledge, the precise effects of diabetic pregnancy on the key processes of kidney development are unclear due to a paucity of studies and limitations in previously used methodologies. The purpose of the present study was to elucidate the effects of hyperglycemia on ureteric branching morphogenesis and nephrogenesis using unbiased techniques. Diabetes was induced in pregnant C57Bl/6J mice using multiple doses of streptozotocin (STZ) on embryonic days (E) 6.5-8.5. Branching morphogenesis was quantified ex vivo using Optical Projection Tomography, and nephrons were counted using unbiased stereology. Maternal hyperglycemia was recognised from E12.5. At E14.5, offspring of diabetic mice demonstrated fetal growth restriction and a marked deficit in ureteric tip number (control 283.7 ± 23.3 vs. STZ 153.2 ± 24.6, mean ± SEM, p<0.01) and ureteric tree length (control 33.1 ± 2.6 mm vs. STZ 17.6 ± 2.7 mm, p = 0.001) vs. controls. At E18.5, fetal growth restriction was still present in offspring of STZ dams and a deficit in nephron endowment was observed (control 1246.2 ± 64.9 vs. STZ 822.4 ± 74.0, p<0.001). Kidney malformations in the form of duplex ureter and hydroureter were a common observation (26%) in embryos of diabetic pregnancy compared with controls (0%). Maternal insulin treatment from E13.5 normalised maternal glycaemia but did not normalise fetal weight nor prevent the nephron deficit. The detrimental effect of hyperglycemia on ureteric branching morphogenesis and, in turn, nephron endowment in the growth-restricted fetus highlights the importance of glycemic control in early gestation and during the initial stages of renal development.Stacey N HokkeJames A ArmitageVictor G PuellesKieran M ShortLynelle JonesIan M SmythJohn F BertramLuise A Cullen-McEwenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58243 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stacey N Hokke James A Armitage Victor G Puelles Kieran M Short Lynelle Jones Ian M Smyth John F Bertram Luise A Cullen-McEwen Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.
description	There is strong evidence from human and animal models that exposure to maternal hyperglycemia during in utero development can detrimentally affect fetal kidney development. Notwithstanding this knowledge, the precise effects of diabetic pregnancy on the key processes of kidney development are unclear due to a paucity of studies and limitations in previously used methodologies. The purpose of the present study was to elucidate the effects of hyperglycemia on ureteric branching morphogenesis and nephrogenesis using unbiased techniques. Diabetes was induced in pregnant C57Bl/6J mice using multiple doses of streptozotocin (STZ) on embryonic days (E) 6.5-8.5. Branching morphogenesis was quantified ex vivo using Optical Projection Tomography, and nephrons were counted using unbiased stereology. Maternal hyperglycemia was recognised from E12.5. At E14.5, offspring of diabetic mice demonstrated fetal growth restriction and a marked deficit in ureteric tip number (control 283.7 ± 23.3 vs. STZ 153.2 ± 24.6, mean ± SEM, p<0.01) and ureteric tree length (control 33.1 ± 2.6 mm vs. STZ 17.6 ± 2.7 mm, p = 0.001) vs. controls. At E18.5, fetal growth restriction was still present in offspring of STZ dams and a deficit in nephron endowment was observed (control 1246.2 ± 64.9 vs. STZ 822.4 ± 74.0, p<0.001). Kidney malformations in the form of duplex ureter and hydroureter were a common observation (26%) in embryos of diabetic pregnancy compared with controls (0%). Maternal insulin treatment from E13.5 normalised maternal glycaemia but did not normalise fetal weight nor prevent the nephron deficit. The detrimental effect of hyperglycemia on ureteric branching morphogenesis and, in turn, nephron endowment in the growth-restricted fetus highlights the importance of glycemic control in early gestation and during the initial stages of renal development.
format	article
author	Stacey N Hokke James A Armitage Victor G Puelles Kieran M Short Lynelle Jones Ian M Smyth John F Bertram Luise A Cullen-McEwen
author_facet	Stacey N Hokke James A Armitage Victor G Puelles Kieran M Short Lynelle Jones Ian M Smyth John F Bertram Luise A Cullen-McEwen
author_sort	Stacey N Hokke
title	Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.
title_short	Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.
title_full	Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.
title_fullStr	Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.
title_full_unstemmed	Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.
title_sort	altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/354f71efdc2e42c79410921dbb514e3b
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Altered ureteric branching morphogenesis and nephron endowment in offspring of diabetic and insulin-treated pregnancy.

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