Electrofusion of single cells in picoliter droplets

Abstract We present a microfluidic chip that enables electrofusion of cells in microdroplets, with exchange of nuclear components. It is shown, to our knowledge for the first time, electrofusion of two HL60 cells, inside a microdroplet. This is the crucial intermediate step for controlled hybridoma...

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Autores principales: Rogier M. Schoeman, Wesley T. E. van den Beld, Evelien W. M. Kemna, Floor Wolbers, Jan C. T. Eijkel, Albert van den Berg
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/3606b79671de4d729848e07db56bd288
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