Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Cas12a is a RNA-guided DNA endonuclease whose detailed mechanisms of target searching and DNA cleavage remained unclear. Here authors use single-molecule fluorescence assays to show that Cas12a searches for their on-target site.

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Autores principales: Yongmoon Jeon, You Hee Choi, Yunsu Jang, Jihyeon Yu, Jiyoung Goo, Gyejun Lee, You Kyeong Jeong, Seung Hwan Lee, In-San Kim, Jin-Soo Kim, Cherlhyun Jeong, Sanghwa Lee, Sangsu Bae
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Science
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Acceso en línea:https://doaj.org/article/36b919cfb25a4a81bcb012e958a2c5c3
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https://doaj.org/article/36b919cfb25a4a81bcb012e958a2c5c3

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