Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

Cas12a is a RNA-guided DNA endonuclease whose detailed mechanisms of target searching and DNA cleavage remained unclear. Here authors use single-molecule fluorescence assays to show that Cas12a searches for their on-target site.

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Autores principales:	Yongmoon Jeon, You Hee Choi, Yunsu Jang, Jihyeon Yu, Jiyoung Goo, Gyejun Lee, You Kyeong Jeong, Seung Hwan Lee, In-San Kim, Jin-Soo Kim, Cherlhyun Jeong, Sanghwa Lee, Sangsu Bae
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/36b919cfb25a4a81bcb012e958a2c5c3
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spelling	oai:doaj.org-article:36b919cfb25a4a81bcb012e958a2c5c32021-12-02T14:40:09ZDirect observation of DNA target searching and cleavage by CRISPR-Cas12a10.1038/s41467-018-05245-x2041-1723https://doaj.org/article/36b919cfb25a4a81bcb012e958a2c5c32018-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05245-xhttps://doaj.org/toc/2041-1723Cas12a is a RNA-guided DNA endonuclease whose detailed mechanisms of target searching and DNA cleavage remained unclear. Here authors use single-molecule fluorescence assays to show that Cas12a searches for their on-target site.Yongmoon JeonYou Hee ChoiYunsu JangJihyeon YuJiyoung GooGyejun LeeYou Kyeong JeongSeung Hwan LeeIn-San KimJin-Soo KimCherlhyun JeongSanghwa LeeSangsu BaeNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yongmoon Jeon You Hee Choi Yunsu Jang Jihyeon Yu Jiyoung Goo Gyejun Lee You Kyeong Jeong Seung Hwan Lee In-San Kim Jin-Soo Kim Cherlhyun Jeong Sanghwa Lee Sangsu Bae Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
description	Cas12a is a RNA-guided DNA endonuclease whose detailed mechanisms of target searching and DNA cleavage remained unclear. Here authors use single-molecule fluorescence assays to show that Cas12a searches for their on-target site.
format	article
author	Yongmoon Jeon You Hee Choi Yunsu Jang Jihyeon Yu Jiyoung Goo Gyejun Lee You Kyeong Jeong Seung Hwan Lee In-San Kim Jin-Soo Kim Cherlhyun Jeong Sanghwa Lee Sangsu Bae
author_facet	Yongmoon Jeon You Hee Choi Yunsu Jang Jihyeon Yu Jiyoung Goo Gyejun Lee You Kyeong Jeong Seung Hwan Lee In-San Kim Jin-Soo Kim Cherlhyun Jeong Sanghwa Lee Sangsu Bae
author_sort	Yongmoon Jeon
title	Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
title_short	Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
title_full	Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
title_fullStr	Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
title_full_unstemmed	Direct observation of DNA target searching and cleavage by CRISPR-Cas12a
title_sort	direct observation of dna target searching and cleavage by crispr-cas12a
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/36b919cfb25a4a81bcb012e958a2c5c3
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Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

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