Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.

The release of the main vasodilator nitric oxide (NO) by the endothelial NO synthase (eNOS) is a hallmark of endothelial function. We aim at elucidating the underlying mechanism how eNOS activity depends on cortical stiffness (К(cortex)) of living endothelial cells. It is hypothesized that cortical...

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Autores principales:	Johannes Fels, Pia Jeggle, Kristina Kusche-Vihrog, Hans Oberleithner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/36fc7a35d50943c4bcbf5e66c5ce99bb
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spelling	oai:doaj.org-article:36fc7a35d50943c4bcbf5e66c5ce99bb2021-11-18T07:11:30ZCortical actin nanodynamics determines nitric oxide release in vascular endothelium.1932-620310.1371/journal.pone.0041520https://doaj.org/article/36fc7a35d50943c4bcbf5e66c5ce99bb2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22844486/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The release of the main vasodilator nitric oxide (NO) by the endothelial NO synthase (eNOS) is a hallmark of endothelial function. We aim at elucidating the underlying mechanism how eNOS activity depends on cortical stiffness (К(cortex)) of living endothelial cells. It is hypothesized that cortical actin dynamics determines К(cortex) and directly influences eNOS activity. By combined atomic force microscopy and fluorescence imaging we generated mechanical and optical sections of single living cells. This approach allows the discrimination between К(cortex) and bulk cell stiffness (К(bulk)) and, additionally, the simultaneous analysis of submembranous actin web dynamics. We show that К(cortex) softens when cortical F-actin depolymerizes and that this shift from a gel-like stiff cortex to a soft G-actin rich layer, triggers the stiffness-sensitive eNOS activity. The results implicate that stiffness changes in the ∼100 nm phase of the submembranous actin web, without affecting К(bulk), regulate NO release and thus determines endothelial function.Johannes FelsPia JeggleKristina Kusche-VihrogHans OberleithnerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e41520 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johannes Fels Pia Jeggle Kristina Kusche-Vihrog Hans Oberleithner Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.
description	The release of the main vasodilator nitric oxide (NO) by the endothelial NO synthase (eNOS) is a hallmark of endothelial function. We aim at elucidating the underlying mechanism how eNOS activity depends on cortical stiffness (К(cortex)) of living endothelial cells. It is hypothesized that cortical actin dynamics determines К(cortex) and directly influences eNOS activity. By combined atomic force microscopy and fluorescence imaging we generated mechanical and optical sections of single living cells. This approach allows the discrimination between К(cortex) and bulk cell stiffness (К(bulk)) and, additionally, the simultaneous analysis of submembranous actin web dynamics. We show that К(cortex) softens when cortical F-actin depolymerizes and that this shift from a gel-like stiff cortex to a soft G-actin rich layer, triggers the stiffness-sensitive eNOS activity. The results implicate that stiffness changes in the ∼100 nm phase of the submembranous actin web, without affecting К(bulk), regulate NO release and thus determines endothelial function.
format	article
author	Johannes Fels Pia Jeggle Kristina Kusche-Vihrog Hans Oberleithner
author_facet	Johannes Fels Pia Jeggle Kristina Kusche-Vihrog Hans Oberleithner
author_sort	Johannes Fels
title	Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.
title_short	Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.
title_full	Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.
title_fullStr	Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.
title_full_unstemmed	Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.
title_sort	cortical actin nanodynamics determines nitric oxide release in vascular endothelium.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/36fc7a35d50943c4bcbf5e66c5ce99bb
work_keys_str_mv	AT johannesfels corticalactinnanodynamicsdeterminesnitricoxidereleaseinvascularendothelium AT piajeggle corticalactinnanodynamicsdeterminesnitricoxidereleaseinvascularendothelium AT kristinakuschevihrog corticalactinnanodynamicsdeterminesnitricoxidereleaseinvascularendothelium AT hansoberleithner corticalactinnanodynamicsdeterminesnitricoxidereleaseinvascularendothelium
_version_	1718423779823058944

Cortical actin nanodynamics determines nitric oxide release in vascular endothelium.

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