A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
Strain engineering is increasingly the bottleneck step in synthetic biology workflows. Here the authors present GTR-CRISPR for rapid, multiplexed engineering of yeast metabolic pathways.
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Autores principales: | , , , , , , |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2019
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Materias: | |
Acceso en línea: | https://doaj.org/article/376af7d923074f22a079f38323a6a87a |
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