A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae

Strain engineering is increasingly the bottleneck step in synthetic biology workflows. Here the authors present GTR-CRISPR for rapid, multiplexed engineering of yeast metabolic pathways.

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Autores principales: Yueping Zhang, Juan Wang, Zibai Wang, Yiming Zhang, Shuobo Shi, Jens Nielsen, Zihe Liu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/376af7d923074f22a079f38323a6a87a
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spelling oai:doaj.org-article:376af7d923074f22a079f38323a6a87a2021-12-02T16:58:03ZA gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae10.1038/s41467-019-09005-32041-1723https://doaj.org/article/376af7d923074f22a079f38323a6a87a2019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09005-3https://doaj.org/toc/2041-1723Strain engineering is increasingly the bottleneck step in synthetic biology workflows. Here the authors present GTR-CRISPR for rapid, multiplexed engineering of yeast metabolic pathways.Yueping ZhangJuan WangZibai WangYiming ZhangShuobo ShiJens NielsenZihe LiuNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Yueping Zhang
Juan Wang
Zibai Wang
Yiming Zhang
Shuobo Shi
Jens Nielsen
Zihe Liu
A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
description Strain engineering is increasingly the bottleneck step in synthetic biology workflows. Here the authors present GTR-CRISPR for rapid, multiplexed engineering of yeast metabolic pathways.
format article
author Yueping Zhang
Juan Wang
Zibai Wang
Yiming Zhang
Shuobo Shi
Jens Nielsen
Zihe Liu
author_facet Yueping Zhang
Juan Wang
Zibai Wang
Yiming Zhang
Shuobo Shi
Jens Nielsen
Zihe Liu
author_sort Yueping Zhang
title A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
title_short A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
title_full A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
title_fullStr A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
title_full_unstemmed A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae
title_sort grna-trna array for crispr-cas9 based rapid multiplexed genome editing in saccharomyces cerevisiae
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/376af7d923074f22a079f38323a6a87a
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