Stool sampling and DNA isolation kits affect DNA quality and bacterial composition following 16S rRNA gene sequencing using MiSeq Illumina platform

Abstract Many studies correlate changes in human gut microbiome with the onset of various diseases, mostly by 16S rRNA gene sequencing. Setting up the optimal sampling and DNA isolation procedures is crucial for robustness and reproducibility of the results. We performed a systematic comparison of s...

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Autores principales: Petra Videnska, Kristyna Smerkova, Barbora Zwinsova, Vlad Popovici, Lenka Micenkova, Karel Sedlar, Eva Budinska
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/37d469f2166f4732bf37c9877781576e
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