Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.

The paternally expressed gene PEG10 is a retrotransposon derived gene adapted through mammalian evolution located on human chromosome 7q21. PEG10 codes for at least two proteins, PEG10-RF1 and PEG10-RF1/2, by -1 frameshift translation. Overexpression or reinduced PEG10 expression was seen in maligna...

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Autores principales:	Heike Lux, Heiko Flammann, Mathias Hafner, Andreas Lux
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3863372bcb0743a085521b7d5eb9d63d
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spelling	oai:doaj.org-article:3863372bcb0743a085521b7d5eb9d63d2021-11-25T06:26:46ZGenetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.1932-620310.1371/journal.pone.0008686https://doaj.org/article/3863372bcb0743a085521b7d5eb9d63d2010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20084274/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The paternally expressed gene PEG10 is a retrotransposon derived gene adapted through mammalian evolution located on human chromosome 7q21. PEG10 codes for at least two proteins, PEG10-RF1 and PEG10-RF1/2, by -1 frameshift translation. Overexpression or reinduced PEG10 expression was seen in malignancies, like hepatocellular carcinoma or B-cell acute and chronic lymphocytic leukemia. PEG10 was also shown to promote adipocyte differentiation. Experimental evidence suggests that the PEG10-RF1 protein is an inhibitor of apoptosis and mediates cell proliferation. Here we present new data on the genomic organization of PEG10 by identifying the major transcription start site, a new splice variant and report the cloning and analysis of 1.9 kb of the PEG10 promoter. Furthermore, we show for the first time that PEG10 translation is initiated at a non-AUG start codon upstream of the previously predicted AUG codon as well as at the AUG codon. The finding that PEG10 translation is initiated at different sides adds a new aspect to the already interesting feature of PEG10's -1 frameshift translation mechanism. It is now important to unravel the cellular functions of the PEG10 protein variants and how they are related to normal or pathological conditions. The generated promoter-reporter constructs can be used for future studies to investigate how PEG10 expression is regulated. In summary, our study provides new data on the genomic organization as well as expression and translation of PEG10, a prerequisite in order to study and understand the role of PEG10 in cancer, embryonic development and normal cell homeostasis.Heike LuxHeiko FlammannMathias HafnerAndreas LuxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8686 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Heike Lux Heiko Flammann Mathias Hafner Andreas Lux Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
description	The paternally expressed gene PEG10 is a retrotransposon derived gene adapted through mammalian evolution located on human chromosome 7q21. PEG10 codes for at least two proteins, PEG10-RF1 and PEG10-RF1/2, by -1 frameshift translation. Overexpression or reinduced PEG10 expression was seen in malignancies, like hepatocellular carcinoma or B-cell acute and chronic lymphocytic leukemia. PEG10 was also shown to promote adipocyte differentiation. Experimental evidence suggests that the PEG10-RF1 protein is an inhibitor of apoptosis and mediates cell proliferation. Here we present new data on the genomic organization of PEG10 by identifying the major transcription start site, a new splice variant and report the cloning and analysis of 1.9 kb of the PEG10 promoter. Furthermore, we show for the first time that PEG10 translation is initiated at a non-AUG start codon upstream of the previously predicted AUG codon as well as at the AUG codon. The finding that PEG10 translation is initiated at different sides adds a new aspect to the already interesting feature of PEG10's -1 frameshift translation mechanism. It is now important to unravel the cellular functions of the PEG10 protein variants and how they are related to normal or pathological conditions. The generated promoter-reporter constructs can be used for future studies to investigate how PEG10 expression is regulated. In summary, our study provides new data on the genomic organization as well as expression and translation of PEG10, a prerequisite in order to study and understand the role of PEG10 in cancer, embryonic development and normal cell homeostasis.
format	article
author	Heike Lux Heiko Flammann Mathias Hafner Andreas Lux
author_facet	Heike Lux Heiko Flammann Mathias Hafner Andreas Lux
author_sort	Heike Lux
title	Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
title_short	Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
title_full	Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
title_fullStr	Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
title_full_unstemmed	Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.
title_sort	genetic and molecular analyses of peg10 reveal new aspects of genomic organization, transcription and translation.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/3863372bcb0743a085521b7d5eb9d63d
work_keys_str_mv	AT heikelux geneticandmolecularanalysesofpeg10revealnewaspectsofgenomicorganizationtranscriptionandtranslation AT heikoflammann geneticandmolecularanalysesofpeg10revealnewaspectsofgenomicorganizationtranscriptionandtranslation AT mathiashafner geneticandmolecularanalysesofpeg10revealnewaspectsofgenomicorganizationtranscriptionandtranslation AT andreaslux geneticandmolecularanalysesofpeg10revealnewaspectsofgenomicorganizationtranscriptionandtranslation
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Genetic and molecular analyses of PEG10 reveal new aspects of genomic organization, transcription and translation.

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