Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration
Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 10000...
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Dove Medical Press
2020
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oai:doaj.org-article:38f66cb3d5484835bfc3ead362c0c1d92021-12-02T10:32:44ZDown-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration1178-7007https://doaj.org/article/38f66cb3d5484835bfc3ead362c0c1d92020-08-01T00:00:00Zhttps://www.dovepress.com/down-regulated-mir-21-in-gestational-diabetes-mellitus-placenta-induce-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 100005, People’s Republic of China; 3Haidian Maternal & Child Health Hospital, Beijing 100080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xu Ma; Hong-Fei XiaReproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of ChinaTel +86-10-62178932Fax +86-10-62179059Email xuma_genetics@163.com; hongfeixia@126.comPurpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats.Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.Keywords: gestational diabetes mellitus, miRNA-21, insulin resistance, PPAR-αGuan CYTian SCao JLWang XQMa XXia HFDove Medical Pressarticlegestational diabetes mellitusmirna-21insulin resistanceppar-αSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 3009-3034 (2020) |
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gestational diabetes mellitus mirna-21 insulin resistance ppar-α Specialties of internal medicine RC581-951 |
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gestational diabetes mellitus mirna-21 insulin resistance ppar-α Specialties of internal medicine RC581-951 Guan CY Tian S Cao JL Wang XQ Ma X Xia HF Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
| description |
Chun-Yi Guan,1,2,* Shi Tian,3,* Jing-Li Cao,1,2 Xue-Qin Wang,1,2 Xu Ma,1,2 Hong-Fei Xia1,2 1Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of China; 2Graduate School, Peking Union Medical College, Beijing Province 100005, People’s Republic of China; 3Haidian Maternal & Child Health Hospital, Beijing 100080, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xu Ma; Hong-Fei XiaReproductive and Genetic Center of National Research Institute for Family Planning, Beijing 100081, People’s Republic of ChinaTel +86-10-62178932Fax +86-10-62179059Email xuma_genetics@163.com; hongfeixia@126.comPurpose: This study aimed to investigate the role of miR-21 expression in the reduction of placental function in GDM patients.Materials and Methods: qRT-PCR was used to detect the differential expression of miR-21 in the serum of gestational diabetes mellitus (GDM) and normal pregnant women, and to verify the functional target gene PPAR-α of miR-21 by double fluorescence experiments. Cellular experiments were performed to verify the effect of PPAR-α on cell function.Results: miR-21 is down-regulated in the serum and placenta of GDM patients compared to normal pregnant women. In the case of insulin resistance, miR-21-5p knockdown promoted glucose uptake, but no significant effect was found under physiological condition. Functional studies have shown that reduced PPAR-α expression can restore miR-21 knockdown-mediated cell growth and metastasis inhibition. Additionally, decreased expression of miR-21 but increased expression of -PPAR-α was observed in patients with GDM and GDM rats.Conclusion: The expression of the placental miR-21-5p, which inhibits cell growth and infiltration by up-regulating PPAR-α, is downregulated in pregnant GDM patients, which in turn may affect the placental function.Keywords: gestational diabetes mellitus, miRNA-21, insulin resistance, PPAR-α |
| format |
article |
| author |
Guan CY Tian S Cao JL Wang XQ Ma X Xia HF |
| author_facet |
Guan CY Tian S Cao JL Wang XQ Ma X Xia HF |
| author_sort |
Guan CY |
| title |
Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
| title_short |
Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
| title_full |
Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
| title_fullStr |
Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
| title_full_unstemmed |
Down-Regulated miR-21 in Gestational Diabetes Mellitus Placenta Induces PPAR-α to Inhibit Cell Proliferation and Infiltration |
| title_sort |
down-regulated mir-21 in gestational diabetes mellitus placenta induces ppar-α to inhibit cell proliferation and infiltration |
| publisher |
Dove Medical Press |
| publishDate |
2020 |
| url |
https://doaj.org/article/38f66cb3d5484835bfc3ead362c0c1d9 |
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