Molecular Characteristics and Antimicrobial Resistance of Salmonella enterica Serovar Schwarzengrund from Chicken Meat in Japan

Our previous study revealed that Salmonella enterica serovar Schwarzengrund-contaminated areas of broiler chickens have expanded from West Japan to East Japan. The present study investigated the antimicrobial resistance and molecular characteristics of 124 S. Sc...

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Autores principales:	Kaoru Matsui, Chisato Nakazawa, Shwe Thiri Maung Maung Khin, Eriko Iwabuchi, Tetsuo Asai, Kanako Ishihara
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	class 1 integron foodborne pathogen multilocus sequence typing <i>Salmonella enterica</i> serovar Schwarzengrund Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/3956c524a8dd45dfbd2d711db49889c7
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spelling	oai:doaj.org-article:3956c524a8dd45dfbd2d711db49889c72021-11-25T16:23:01ZMolecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan10.3390/antibiotics101113362079-6382https://doaj.org/article/3956c524a8dd45dfbd2d711db49889c72021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1336https://doaj.org/toc/2079-6382Our previous study revealed that <i>Salmonella enterica</i> serovar Schwarzengrund-contaminated areas of broiler chickens have expanded from West Japan to East Japan. The present study investigated the antimicrobial resistance and molecular characteristics of 124 <i>S.</i> Schwarzengrund isolates obtained from chicken meat produced in East and West Japan from 2008 to 2019. Comparing the isolates obtained in 2008 and 2015–2019, an increase in the proportion of those resistant to kanamycin [51.4–89.7% (<i>p</i> < 0.001)] was observed. In contrast, the proportion of isolates resistant to both streptomycin and tetracycline and those that harbored a 1.0-kb class 1 integron, <i>aadA1</i>, and <i>tetA</i>, significantly decreased from 100% in 2008 to 47.1% in 2015–2019 (<i>p</i> < 0.001). A 1.0-kb class 1 integron containing <i>aadA1</i>, harbored by 78 isolates, was different from that reported in globally distributed <i>S.</i> Schwarzengrund strains (1.9 kb, containing the <i>dfrA12-aadA2</i> gene cassette). Twenty-five isolates from different product districts and years of isolation were typed as sequence type (ST) 241 with multilocus sequence typing. Our results suggest that <i>S.</i> Schwarzengrund, which contaminates chicken meat in Japan, shares a common ancestor regardless of the product district from 2008 to recent years. Moreover, <i>S.</i> Schwarzengrund ST241 may have spread from western to eastern Japan.Kaoru MatsuiChisato NakazawaShwe Thiri Maung Maung KhinEriko IwabuchiTetsuo AsaiKanako IshiharaMDPI AGarticleclass 1 integronfoodborne pathogenmultilocus sequence typing<i>Salmonella enterica</i> serovar SchwarzengrundTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1336, p 1336 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	class 1 integron foodborne pathogen multilocus sequence typing <i>Salmonella enterica</i> serovar Schwarzengrund Therapeutics. Pharmacology RM1-950
spellingShingle	class 1 integron foodborne pathogen multilocus sequence typing <i>Salmonella enterica</i> serovar Schwarzengrund Therapeutics. Pharmacology RM1-950 Kaoru Matsui Chisato Nakazawa Shwe Thiri Maung Maung Khin Eriko Iwabuchi Tetsuo Asai Kanako Ishihara Molecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan
description	Our previous study revealed that <i>Salmonella enterica</i> serovar Schwarzengrund-contaminated areas of broiler chickens have expanded from West Japan to East Japan. The present study investigated the antimicrobial resistance and molecular characteristics of 124 <i>S.</i> Schwarzengrund isolates obtained from chicken meat produced in East and West Japan from 2008 to 2019. Comparing the isolates obtained in 2008 and 2015–2019, an increase in the proportion of those resistant to kanamycin [51.4–89.7% (<i>p</i> < 0.001)] was observed. In contrast, the proportion of isolates resistant to both streptomycin and tetracycline and those that harbored a 1.0-kb class 1 integron, <i>aadA1</i>, and <i>tetA</i>, significantly decreased from 100% in 2008 to 47.1% in 2015–2019 (<i>p</i> < 0.001). A 1.0-kb class 1 integron containing <i>aadA1</i>, harbored by 78 isolates, was different from that reported in globally distributed <i>S.</i> Schwarzengrund strains (1.9 kb, containing the <i>dfrA12-aadA2</i> gene cassette). Twenty-five isolates from different product districts and years of isolation were typed as sequence type (ST) 241 with multilocus sequence typing. Our results suggest that <i>S.</i> Schwarzengrund, which contaminates chicken meat in Japan, shares a common ancestor regardless of the product district from 2008 to recent years. Moreover, <i>S.</i> Schwarzengrund ST241 may have spread from western to eastern Japan.
format	article
author	Kaoru Matsui Chisato Nakazawa Shwe Thiri Maung Maung Khin Eriko Iwabuchi Tetsuo Asai Kanako Ishihara
author_facet	Kaoru Matsui Chisato Nakazawa Shwe Thiri Maung Maung Khin Eriko Iwabuchi Tetsuo Asai Kanako Ishihara
author_sort	Kaoru Matsui
title	Molecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan
title_short	Molecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan
title_full	Molecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan
title_fullStr	Molecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan
title_full_unstemmed	Molecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan
title_sort	molecular characteristics and antimicrobial resistance of <i>salmonella enterica</i> serovar schwarzengrund from chicken meat in japan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/3956c524a8dd45dfbd2d711db49889c7
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Molecular Characteristics and Antimicrobial Resistance of <i>Salmonella enterica</i> Serovar Schwarzengrund from Chicken Meat in Japan

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