CRISPR technologies and the search for the PAM-free nuclease

One of the key limitations of CRISPR-Cas-based genome editing techniques is the PAM dependency. Here, the authors review ongoing efforts towards realizing PAM-free nucleases, address potential consequences of eliminating PAM recognition, and propose an alternative nuclease repertoire covering all po...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Daphne Collias, Chase L. Beisel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/3b0005fc19784851be7b5e4a5d35393a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3b0005fc19784851be7b5e4a5d35393a
record_format	dspace
spelling	oai:doaj.org-article:3b0005fc19784851be7b5e4a5d35393a2021-12-02T13:57:11ZCRISPR technologies and the search for the PAM-free nuclease10.1038/s41467-020-20633-y2041-1723https://doaj.org/article/3b0005fc19784851be7b5e4a5d35393a2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-20633-yhttps://doaj.org/toc/2041-1723One of the key limitations of CRISPR-Cas-based genome editing techniques is the PAM dependency. Here, the authors review ongoing efforts towards realizing PAM-free nucleases, address potential consequences of eliminating PAM recognition, and propose an alternative nuclease repertoire covering all possible PAM sequences.Daphne ColliasChase L. BeiselNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Daphne Collias Chase L. Beisel CRISPR technologies and the search for the PAM-free nuclease
description	One of the key limitations of CRISPR-Cas-based genome editing techniques is the PAM dependency. Here, the authors review ongoing efforts towards realizing PAM-free nucleases, address potential consequences of eliminating PAM recognition, and propose an alternative nuclease repertoire covering all possible PAM sequences.
format	article
author	Daphne Collias Chase L. Beisel
author_facet	Daphne Collias Chase L. Beisel
author_sort	Daphne Collias
title	CRISPR technologies and the search for the PAM-free nuclease
title_short	CRISPR technologies and the search for the PAM-free nuclease
title_full	CRISPR technologies and the search for the PAM-free nuclease
title_fullStr	CRISPR technologies and the search for the PAM-free nuclease
title_full_unstemmed	CRISPR technologies and the search for the PAM-free nuclease
title_sort	crispr technologies and the search for the pam-free nuclease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/3b0005fc19784851be7b5e4a5d35393a
work_keys_str_mv	AT daphnecollias crisprtechnologiesandthesearchforthepamfreenuclease AT chaselbeisel crisprtechnologiesandthesearchforthepamfreenuclease
_version_	1718392314968145920

CRISPR technologies and the search for the PAM-free nuclease

Ejemplares similares