Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630
Abstract The pandemic of 2019 caused by the novel coronavirus (SARS-CoV-2) is still rapidly spreading worldwide. Nucleic acid amplification serves as the gold standard method for confirmation of COVID-19 infection. However, challenges faced for diagnostic laboratories from undeveloped countries incl...
Saved in:
Main Authors: | Alejandro Castellanos-Gonzalez, Thomas R. Shelite, Nicole Lloyd, Aygul Sadiqova, Ren Ping, Natalie Williams-Bouyer, Peter C. Melby, Bruno L. Travi |
---|---|
Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
|
Subjects: | |
Online Access: | https://doaj.org/article/3c7d2cb5aef941e292e49bff2783e8c4 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Selection of stable reference genes for RT-qPCR in Rhodococcus opacus PD630
by: Drew M. DeLorenzo, et al.
Published: (2018) -
Multiplex detection of bacteria on an integrated centrifugal disk using bead-beating lysis and loop-mediated amplification
by: He Yan, et al.
Published: (2017) -
An enhanced isothermal amplification assay for viral detection
by: Jason Qian, et al.
Published: (2020) -
Amplification by PCR artificially reduces the proportion of the rare biosphere in microbial communities.
by: Juan M Gonzalez, et al.
Published: (2012) -
Prediction of PCR amplification from primer and template sequences using recurrent neural network
by: Kotetsu Kayama, et al.
Published: (2021)