Direct RT-PCR amplification of SARS-CoV-2 from clinical samples using a concentrated viral lysis-amplification buffer prepared with IGEPAL-630

Abstract The pandemic of 2019 caused by the novel coronavirus (SARS-CoV-2) is still rapidly spreading worldwide. Nucleic acid amplification serves as the gold standard method for confirmation of COVID-19 infection. However, challenges faced for diagnostic laboratories from undeveloped countries incl...

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Bibliographic Details
Main Authors: Alejandro Castellanos-Gonzalez, Thomas R. Shelite, Nicole Lloyd, Aygul Sadiqova, Ren Ping, Natalie Williams-Bouyer, Peter C. Melby, Bruno L. Travi
Format: article
Language:EN
Published: Nature Portfolio 2021
Subjects:
R
Q
Online Access:https://doaj.org/article/3c7d2cb5aef941e292e49bff2783e8c4
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