Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.

Although docosahexaenoic acid (DHA), an important dietary omega-3 polyunsaturated fatty acid (PUFA), is at present primarily sourced from marine fish, bioengineered crops producing DHA may offer a more sustainable and cost-effective source. DHA has been produced in transgenic oilseed crops, however,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Laura L Wayne, Daniel J Gachotte, Paul R Graupner, Yelena Adelfinskaya, David G McCaskill, James G Metz, Ross Zirkle, Terence A Walsh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3caa47455514475396fed9df3c7764c5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:3caa47455514475396fed9df3c7764c5
record_format	dspace
spelling	oai:doaj.org-article:3caa47455514475396fed9df3c7764c52021-12-02T20:19:33ZPlant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.1932-620310.1371/journal.pone.0256625https://doaj.org/article/3caa47455514475396fed9df3c7764c52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256625https://doaj.org/toc/1932-6203Although docosahexaenoic acid (DHA), an important dietary omega-3 polyunsaturated fatty acid (PUFA), is at present primarily sourced from marine fish, bioengineered crops producing DHA may offer a more sustainable and cost-effective source. DHA has been produced in transgenic oilseed crops, however, DHA in seed oil primarily occupies the sn-1/3 positions of triacylglycerol (TAG) with relatively low amounts of DHA in the sn-2 position. To increase the amount of DHA in the sn-2 position of TAG and in seed oil, putative lysophosphatidic acid acyltransferases (LPAATs) were identified and characterized from the DHA-producing alga Schizochytrium sp. and from soybean (Glycine max). The affinity-purified proteins were confirmed to have LPAAT activity. Expression of the Schizochytrium or soybean LPAATs in DHA-producing Arabidopsis expressing the Schizochytrium PUFA synthase system significantly increased the total amount of DHA in seed oil. A novel sensitive band-selective heteronuclear single quantum coherence (HSQC) NMR method was developed to quantify DHA at the sn-2 position of glycerolipids. More than two-fold increases in sn-2 DHA were observed for Arabidopsis lines expressing Schizochytrium or soybean LPAATs, with one Schizochytrium LPAAT driving DHA accumulation in the sn-2 position to 61% of the total DHA. Furthermore, expression of a soybean LPAAT led to a redistribution of DHA-containing TAG species, with two new TAG species identified. Our results demonstrate that transgenic expression of Schizochytrium or soybean LPAATs can increase the proportion of DHA at the sn-2 position of TAG and the total amount of DHA in the seed oil of a DHA-accumulating oilseed plant. Additionally, the band-selective HSQC NMR method that we developed provides a sensitive and robust method for determining the regiochemistry of DHA in glycerolipids. These findings will benefit the advancement of sustainable sources of DHA via transgenic crops such as canola and soybean.Laura L WayneDaniel J GachottePaul R GraupnerYelena AdelfinskayaDavid G McCaskillJames G MetzRoss ZirkleTerence A WalshPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0256625 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura L Wayne Daniel J Gachotte Paul R Graupner Yelena Adelfinskaya David G McCaskill James G Metz Ross Zirkle Terence A Walsh Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.
description	Although docosahexaenoic acid (DHA), an important dietary omega-3 polyunsaturated fatty acid (PUFA), is at present primarily sourced from marine fish, bioengineered crops producing DHA may offer a more sustainable and cost-effective source. DHA has been produced in transgenic oilseed crops, however, DHA in seed oil primarily occupies the sn-1/3 positions of triacylglycerol (TAG) with relatively low amounts of DHA in the sn-2 position. To increase the amount of DHA in the sn-2 position of TAG and in seed oil, putative lysophosphatidic acid acyltransferases (LPAATs) were identified and characterized from the DHA-producing alga Schizochytrium sp. and from soybean (Glycine max). The affinity-purified proteins were confirmed to have LPAAT activity. Expression of the Schizochytrium or soybean LPAATs in DHA-producing Arabidopsis expressing the Schizochytrium PUFA synthase system significantly increased the total amount of DHA in seed oil. A novel sensitive band-selective heteronuclear single quantum coherence (HSQC) NMR method was developed to quantify DHA at the sn-2 position of glycerolipids. More than two-fold increases in sn-2 DHA were observed for Arabidopsis lines expressing Schizochytrium or soybean LPAATs, with one Schizochytrium LPAAT driving DHA accumulation in the sn-2 position to 61% of the total DHA. Furthermore, expression of a soybean LPAAT led to a redistribution of DHA-containing TAG species, with two new TAG species identified. Our results demonstrate that transgenic expression of Schizochytrium or soybean LPAATs can increase the proportion of DHA at the sn-2 position of TAG and the total amount of DHA in the seed oil of a DHA-accumulating oilseed plant. Additionally, the band-selective HSQC NMR method that we developed provides a sensitive and robust method for determining the regiochemistry of DHA in glycerolipids. These findings will benefit the advancement of sustainable sources of DHA via transgenic crops such as canola and soybean.
format	article
author	Laura L Wayne Daniel J Gachotte Paul R Graupner Yelena Adelfinskaya David G McCaskill James G Metz Ross Zirkle Terence A Walsh
author_facet	Laura L Wayne Daniel J Gachotte Paul R Graupner Yelena Adelfinskaya David G McCaskill James G Metz Ross Zirkle Terence A Walsh
author_sort	Laura L Wayne
title	Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.
title_short	Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.
title_full	Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.
title_fullStr	Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.
title_full_unstemmed	Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.
title_sort	plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic arabidopsis seed oil.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/3caa47455514475396fed9df3c7764c5
work_keys_str_mv	AT lauralwayne plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil AT danieljgachotte plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil AT paulrgraupner plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil AT yelenaadelfinskaya plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil AT davidgmccaskill plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil AT jamesgmetz plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil AT rosszirkle plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil AT terenceawalsh plantandalgallysophosphatidicacidacyltransferasesincreasedocosahexaenoicacidaccumulationatthesn2positionoftriacylglycerolintransgenicarabidopsisseedoil
_version_	1718374160342712320

Plant and algal lysophosphatidic acid acyltransferases increase docosahexaenoic acid accumulation at the sn-2 position of triacylglycerol in transgenic Arabidopsis seed oil.

Ejemplares similares