A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.

A new application of antibodies is to use them as macromolecular chaperones. Protein antigens usually have multiple epitopes, thus, there may be a plurality of antibodies binding to one antigen. However, not all antibodies that bind to one antigen could act as a chaperone. Experiments show that some...

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Autores principales:	Jianyu Feng, Hong Guo, Sen Li, Tun Lu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3cf5c2bd4793473aaf370a78c12b2d79
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spelling	oai:doaj.org-article:3cf5c2bd4793473aaf370a78c12b2d792021-11-18T07:48:00ZA study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.1932-620310.1371/journal.pone.0062147https://doaj.org/article/3cf5c2bd4793473aaf370a78c12b2d792013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23637984/?tool=EBIhttps://doaj.org/toc/1932-6203A new application of antibodies is to use them as macromolecular chaperones. Protein antigens usually have multiple epitopes, thus, there may be a plurality of antibodies binding to one antigen. However, not all antibodies that bind to one antigen could act as a chaperone. Experiments show that some screened anti-human creatine kinase single chain antibodies (scFV) could assist in the folding and stabilizing of the enzyme, while others could not. We built the model of the single chain antibody (scFv-A4) that increased the stability of human creatine kinase (HCK) by the homology modeling method. Epitopes of human creatine kinase were predicted by computer and then the binding of scFv-A4 and HCK was modeled with computer. The calculation results were further combined with the peptide array membrane experiment results to obtain reliable models for the scFv-A4-HCK complex. Based on the above study we gave an explanation about how scFv-A4 could act as a macromolecular chaperone assisting the folding of HCK. This study provides an approach for predicting antigen-antibody binding mode and also a useful theoretical guidance for the study of antibodies' chaperone-like function.Jianyu FengHong GuoSen LiTun LuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e62147 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jianyu Feng Hong Guo Sen Li Tun Lu A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.
description	A new application of antibodies is to use them as macromolecular chaperones. Protein antigens usually have multiple epitopes, thus, there may be a plurality of antibodies binding to one antigen. However, not all antibodies that bind to one antigen could act as a chaperone. Experiments show that some screened anti-human creatine kinase single chain antibodies (scFV) could assist in the folding and stabilizing of the enzyme, while others could not. We built the model of the single chain antibody (scFv-A4) that increased the stability of human creatine kinase (HCK) by the homology modeling method. Epitopes of human creatine kinase were predicted by computer and then the binding of scFv-A4 and HCK was modeled with computer. The calculation results were further combined with the peptide array membrane experiment results to obtain reliable models for the scFv-A4-HCK complex. Based on the above study we gave an explanation about how scFv-A4 could act as a macromolecular chaperone assisting the folding of HCK. This study provides an approach for predicting antigen-antibody binding mode and also a useful theoretical guidance for the study of antibodies' chaperone-like function.
format	article
author	Jianyu Feng Hong Guo Sen Li Tun Lu
author_facet	Jianyu Feng Hong Guo Sen Li Tun Lu
author_sort	Jianyu Feng
title	A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.
title_short	A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.
title_full	A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.
title_fullStr	A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.
title_full_unstemmed	A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.
title_sort	study of the mechanism of the chaperone-like function of an scfv of human creatine kinase by computer simulation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/3cf5c2bd4793473aaf370a78c12b2d79
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A study of the mechanism of the chaperone-like function of an scFv of human creatine kinase by computer simulation.

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