Oligomeric coiled-coil adhesin YadA is a double-edged sword.

Yersinia adhesin A (YadA) is an essential virulence factor for the food-borne pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis. Surprisingly, it is a pseudogene in Yersinia pestis. Even more intriguing, the introduction of a functional yadA gene in Y. pestis EV76 was shown to correl...

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Autores principales: Salome Casutt-Meyer, Francesco Renzi, Mathias Schmaler, Naja J Jann, Marlise Amstutz, Guy R Cornelis
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spelling oai:doaj.org-article:3dc940482e824dd3a9ef48b4352415ed2021-11-18T07:01:58ZOligomeric coiled-coil adhesin YadA is a double-edged sword.1932-620310.1371/journal.pone.0015159https://doaj.org/article/3dc940482e824dd3a9ef48b4352415ed2010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21170337/?tool=EBIhttps://doaj.org/toc/1932-6203Yersinia adhesin A (YadA) is an essential virulence factor for the food-borne pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis. Surprisingly, it is a pseudogene in Yersinia pestis. Even more intriguing, the introduction of a functional yadA gene in Y. pestis EV76 was shown to correlate with a decrease in virulence in a mouse model. Here, we report that wild type (wt) Y. enterocolitica E40, as well as YadA-deprived E40 induced the synthesis of neutrophil extracellular traps (NETs) upon contact with neutrophils, but only YadA-expressing Y. enterocolitica adhered to NETs and were killed. As binding seemed to be a prerequisite for killing, we searched for YadA-binding substrates and detected the presence of collagen within NETs. E40 bacteria expressing V98D,N99A mutant YadA with a severely reduced ability to bind collagen were found to be more resistant to killing, suggesting that collagen binding contributes significantly to sensitivity to NETs. Wt Y. pestis EV76 were resistant to killing by NETs, while recombinant EV76 expressing YadA from either Y. pseudotuberculosis or Y. enterocolitica were sensitive to killing by NETs, outlining the importance of YadA for susceptibility to NET-dependent killing. Recombinant EV76 endowed with YadA from Y. enterocolitica were also less virulent for the mouse than wt EV76, as shown before. In addition, EV76 carrying wt YadA were less virulent for the mouse than EV76 expressing YadA(V₉₈D,N₉₉A). The observation that YadA makes Yersinia sensitive to NETs provides an explanation as for why evolution selected for the inactivation of yadA in the flea-borne Y. pestis and clarifies an old enigma. Since YadA imposes the same cost to the food-borne Yersinia but was nevertheless conserved by evolution, this observation also illustrates the duality of some virulence functions.Salome Casutt-MeyerFrancesco RenziMathias SchmalerNaja J JannMarlise AmstutzGuy R CornelisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15159 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Salome Casutt-Meyer
Francesco Renzi
Mathias Schmaler
Naja J Jann
Marlise Amstutz
Guy R Cornelis
Oligomeric coiled-coil adhesin YadA is a double-edged sword.
description Yersinia adhesin A (YadA) is an essential virulence factor for the food-borne pathogens Yersinia enterocolitica and Yersinia pseudotuberculosis. Surprisingly, it is a pseudogene in Yersinia pestis. Even more intriguing, the introduction of a functional yadA gene in Y. pestis EV76 was shown to correlate with a decrease in virulence in a mouse model. Here, we report that wild type (wt) Y. enterocolitica E40, as well as YadA-deprived E40 induced the synthesis of neutrophil extracellular traps (NETs) upon contact with neutrophils, but only YadA-expressing Y. enterocolitica adhered to NETs and were killed. As binding seemed to be a prerequisite for killing, we searched for YadA-binding substrates and detected the presence of collagen within NETs. E40 bacteria expressing V98D,N99A mutant YadA with a severely reduced ability to bind collagen were found to be more resistant to killing, suggesting that collagen binding contributes significantly to sensitivity to NETs. Wt Y. pestis EV76 were resistant to killing by NETs, while recombinant EV76 expressing YadA from either Y. pseudotuberculosis or Y. enterocolitica were sensitive to killing by NETs, outlining the importance of YadA for susceptibility to NET-dependent killing. Recombinant EV76 endowed with YadA from Y. enterocolitica were also less virulent for the mouse than wt EV76, as shown before. In addition, EV76 carrying wt YadA were less virulent for the mouse than EV76 expressing YadA(V₉₈D,N₉₉A). The observation that YadA makes Yersinia sensitive to NETs provides an explanation as for why evolution selected for the inactivation of yadA in the flea-borne Y. pestis and clarifies an old enigma. Since YadA imposes the same cost to the food-borne Yersinia but was nevertheless conserved by evolution, this observation also illustrates the duality of some virulence functions.
format article
author Salome Casutt-Meyer
Francesco Renzi
Mathias Schmaler
Naja J Jann
Marlise Amstutz
Guy R Cornelis
author_facet Salome Casutt-Meyer
Francesco Renzi
Mathias Schmaler
Naja J Jann
Marlise Amstutz
Guy R Cornelis
author_sort Salome Casutt-Meyer
title Oligomeric coiled-coil adhesin YadA is a double-edged sword.
title_short Oligomeric coiled-coil adhesin YadA is a double-edged sword.
title_full Oligomeric coiled-coil adhesin YadA is a double-edged sword.
title_fullStr Oligomeric coiled-coil adhesin YadA is a double-edged sword.
title_full_unstemmed Oligomeric coiled-coil adhesin YadA is a double-edged sword.
title_sort oligomeric coiled-coil adhesin yada is a double-edged sword.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/3dc940482e824dd3a9ef48b4352415ed
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