Towards the generation of B-cell receptor retrogenic mice.

Transgenic expression of B- and T-cell receptors (BCRs and TCRs, respectively) has been a standard tool to study lymphocyte development and function in vivo. The generation of transgenic mice is time-consuming and, therefore, a faster method to study the biology of defined lymphocyte receptors in vi...

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Autores principales:	Jenny Freitag, Sylvia Heink, Edith Roth, Jürgen Wittmann, Hans-Martin Jäck, Thomas Kamradt
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/3ee9a7f3c7684310a73784dbc8fd9ae3
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spelling	oai:doaj.org-article:3ee9a7f3c7684310a73784dbc8fd9ae32021-11-25T05:57:25ZTowards the generation of B-cell receptor retrogenic mice.1932-620310.1371/journal.pone.0109199https://doaj.org/article/3ee9a7f3c7684310a73784dbc8fd9ae32014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109199https://doaj.org/toc/1932-6203Transgenic expression of B- and T-cell receptors (BCRs and TCRs, respectively) has been a standard tool to study lymphocyte development and function in vivo. The generation of transgenic mice is time-consuming and, therefore, a faster method to study the biology of defined lymphocyte receptors in vivo would be highly welcome. Using 2A peptide-linked multicistronic retroviral vectors to transduce stem cells, TCRs can be expressed rapidly in mice of any background. We aimed at adopting this retrogenic technology to the in vivo expression of BCRs. Using a well characterised BCR specific for hen egg lysozyme (HEL), we achieved surface expression of the retrogenically encoded BCR in a Rag-deficient pro B-cell line in vitro. In vivo, retrogenic BCRs were detectable only intracellularly but not on the surface of B cells from wild type or Rag2-deficient mice. This data, together with the fact that no BCR retrogenic mouse model has been published in the 7 years since the method was originally published for TCRs, strongly suggests that achieving BCR-expression in vivo with retrogenic technology is highly challenging if not impossible.Jenny FreitagSylvia HeinkEdith RothJürgen WittmannHans-Martin JäckThomas KamradtPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109199 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jenny Freitag Sylvia Heink Edith Roth Jürgen Wittmann Hans-Martin Jäck Thomas Kamradt Towards the generation of B-cell receptor retrogenic mice.
description	Transgenic expression of B- and T-cell receptors (BCRs and TCRs, respectively) has been a standard tool to study lymphocyte development and function in vivo. The generation of transgenic mice is time-consuming and, therefore, a faster method to study the biology of defined lymphocyte receptors in vivo would be highly welcome. Using 2A peptide-linked multicistronic retroviral vectors to transduce stem cells, TCRs can be expressed rapidly in mice of any background. We aimed at adopting this retrogenic technology to the in vivo expression of BCRs. Using a well characterised BCR specific for hen egg lysozyme (HEL), we achieved surface expression of the retrogenically encoded BCR in a Rag-deficient pro B-cell line in vitro. In vivo, retrogenic BCRs were detectable only intracellularly but not on the surface of B cells from wild type or Rag2-deficient mice. This data, together with the fact that no BCR retrogenic mouse model has been published in the 7 years since the method was originally published for TCRs, strongly suggests that achieving BCR-expression in vivo with retrogenic technology is highly challenging if not impossible.
format	article
author	Jenny Freitag Sylvia Heink Edith Roth Jürgen Wittmann Hans-Martin Jäck Thomas Kamradt
author_facet	Jenny Freitag Sylvia Heink Edith Roth Jürgen Wittmann Hans-Martin Jäck Thomas Kamradt
author_sort	Jenny Freitag
title	Towards the generation of B-cell receptor retrogenic mice.
title_short	Towards the generation of B-cell receptor retrogenic mice.
title_full	Towards the generation of B-cell receptor retrogenic mice.
title_fullStr	Towards the generation of B-cell receptor retrogenic mice.
title_full_unstemmed	Towards the generation of B-cell receptor retrogenic mice.
title_sort	towards the generation of b-cell receptor retrogenic mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/3ee9a7f3c7684310a73784dbc8fd9ae3
work_keys_str_mv	AT jennyfreitag towardsthegenerationofbcellreceptorretrogenicmice AT sylviaheink towardsthegenerationofbcellreceptorretrogenicmice AT edithroth towardsthegenerationofbcellreceptorretrogenicmice AT jurgenwittmann towardsthegenerationofbcellreceptorretrogenicmice AT hansmartinjack towardsthegenerationofbcellreceptorretrogenicmice AT thomaskamradt towardsthegenerationofbcellreceptorretrogenicmice
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Towards the generation of B-cell receptor retrogenic mice.

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