Single-molecule visualization of conformational changes and substrate transport in the vitamin B12 ABC importer BtuCD-F

The vitamin B12 importer BtuCD-F is a type II ATP-binding cassette transporter. Here the authors use single-molecule fluorescence techniques to analyse ATP hydrolysis and substrate transport in individual BtuCD-F complexes embedded in liposomes, and propose a transport model.

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Autores principales: Joris M. H. Goudsmits, Dirk Jan Slotboom, Antoine M. van Oijen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/403f34f7a6fc4b4897cd19e63f9c11cd
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