An upstream open reading frame modulates ebola virus polymerase translation and virus replication.

Ebolaviruses, highly lethal zoonotic pathogens, possess longer genomes than most other non-segmented negative-strand RNA viruses due in part to long 5' and 3' untranslated regions (UTRs) present in the seven viral transcriptional units. To date, specific functions have not been assigned to...

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Autores principales:	Reed S Shabman, Thomas Hoenen, Allison Groseth, Omar Jabado, Jennifer M Binning, Gaya K Amarasinghe, Heinz Feldmann, Christopher F Basler
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/41183d7040a844a4b4f75e7055516713
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spelling	oai:doaj.org-article:41183d7040a844a4b4f75e70555167132021-11-18T06:06:06ZAn upstream open reading frame modulates ebola virus polymerase translation and virus replication.1553-73661553-737410.1371/journal.ppat.1003147https://doaj.org/article/41183d7040a844a4b4f75e70555167132013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23382680/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Ebolaviruses, highly lethal zoonotic pathogens, possess longer genomes than most other non-segmented negative-strand RNA viruses due in part to long 5' and 3' untranslated regions (UTRs) present in the seven viral transcriptional units. To date, specific functions have not been assigned to these UTRs. With reporter assays, we demonstrated that the Zaire ebolavirus (EBOV) 5'-UTRs lack internal ribosomal entry site function. However, the 5'-UTRs do differentially regulate cap-dependent translation when placed upstream of a GFP reporter gene. Most dramatically, the 5'-UTR derived from the viral polymerase (L) mRNA strongly suppressed translation of GFP compared to a β-actin 5'-UTR. The L 5'-UTR is one of four viral genes to possess upstream AUGs (uAUGs), and ablation of each uAUG enhanced translation of the primary ORF (pORF), most dramatically in the case of the L 5'-UTR. The L uAUG was sufficient to initiate translation, is surrounded by a "weak" Kozak sequence and suppressed pORF translation in a position-dependent manner. Under conditions where eIF2α was phosphorylated, the presence of the uORF maintained translation of the L pORF, indicating that the uORF modulates L translation in response to cellular stress. To directly address the role of the L uAUG in virus replication, a recombinant EBOV was generated in which the L uAUG was mutated to UCG. Strikingly, mutating two nucleotides outside of previously-defined protein coding and cis-acting regulatory sequences attenuated virus growth to titers 10-100-fold lower than a wild-type virus in Vero and A549 cells. The mutant virus also exhibited decreased viral RNA synthesis as early as 6 hours post-infection and enhanced sensitivity to the stress inducer thapsigargin. Cumulatively, these data identify novel mechanisms by which EBOV regulates its polymerase expression, demonstrate their relevance to virus replication and identify a potential therapeutic target.Reed S ShabmanThomas HoenenAllison GrosethOmar JabadoJennifer M BinningGaya K AmarasingheHeinz FeldmannChristopher F BaslerPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 1, p e1003147 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Reed S Shabman Thomas Hoenen Allison Groseth Omar Jabado Jennifer M Binning Gaya K Amarasinghe Heinz Feldmann Christopher F Basler An upstream open reading frame modulates ebola virus polymerase translation and virus replication.
description	Ebolaviruses, highly lethal zoonotic pathogens, possess longer genomes than most other non-segmented negative-strand RNA viruses due in part to long 5' and 3' untranslated regions (UTRs) present in the seven viral transcriptional units. To date, specific functions have not been assigned to these UTRs. With reporter assays, we demonstrated that the Zaire ebolavirus (EBOV) 5'-UTRs lack internal ribosomal entry site function. However, the 5'-UTRs do differentially regulate cap-dependent translation when placed upstream of a GFP reporter gene. Most dramatically, the 5'-UTR derived from the viral polymerase (L) mRNA strongly suppressed translation of GFP compared to a β-actin 5'-UTR. The L 5'-UTR is one of four viral genes to possess upstream AUGs (uAUGs), and ablation of each uAUG enhanced translation of the primary ORF (pORF), most dramatically in the case of the L 5'-UTR. The L uAUG was sufficient to initiate translation, is surrounded by a "weak" Kozak sequence and suppressed pORF translation in a position-dependent manner. Under conditions where eIF2α was phosphorylated, the presence of the uORF maintained translation of the L pORF, indicating that the uORF modulates L translation in response to cellular stress. To directly address the role of the L uAUG in virus replication, a recombinant EBOV was generated in which the L uAUG was mutated to UCG. Strikingly, mutating two nucleotides outside of previously-defined protein coding and cis-acting regulatory sequences attenuated virus growth to titers 10-100-fold lower than a wild-type virus in Vero and A549 cells. The mutant virus also exhibited decreased viral RNA synthesis as early as 6 hours post-infection and enhanced sensitivity to the stress inducer thapsigargin. Cumulatively, these data identify novel mechanisms by which EBOV regulates its polymerase expression, demonstrate their relevance to virus replication and identify a potential therapeutic target.
format	article
author	Reed S Shabman Thomas Hoenen Allison Groseth Omar Jabado Jennifer M Binning Gaya K Amarasinghe Heinz Feldmann Christopher F Basler
author_facet	Reed S Shabman Thomas Hoenen Allison Groseth Omar Jabado Jennifer M Binning Gaya K Amarasinghe Heinz Feldmann Christopher F Basler
author_sort	Reed S Shabman
title	An upstream open reading frame modulates ebola virus polymerase translation and virus replication.
title_short	An upstream open reading frame modulates ebola virus polymerase translation and virus replication.
title_full	An upstream open reading frame modulates ebola virus polymerase translation and virus replication.
title_fullStr	An upstream open reading frame modulates ebola virus polymerase translation and virus replication.
title_full_unstemmed	An upstream open reading frame modulates ebola virus polymerase translation and virus replication.
title_sort	upstream open reading frame modulates ebola virus polymerase translation and virus replication.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/41183d7040a844a4b4f75e7055516713
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An upstream open reading frame modulates ebola virus polymerase translation and virus replication.

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