Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats

BACKGROUND AND OBJECTIVE: Acetaminophen overdose is the most frequent cause of liver injuries. N-acetyl-p-benzoquinone imine (NAPQI) has been proposed as the toxic metabolite of acetaminophen induced by cytochrome P-450. Piperine has antioxidant activities and has been introduced as an inhibitor of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	MT Kazemi, M Pour Nasrollah, MM Rezaei, GH.A Jorsaraei, GH Maliji, S Kazemi, E Zabihi, M Pouramir, AA Moghadamnia
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2012
Materias:	acetaminophen piperine silymarin n-acetyl-p-benzoquinone imine aspartate aminotransferase alanine aminotransferase antioxidant ferric reducing ability of plasma Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/424fecfe063148669e9378e9f9cbf2b6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:424fecfe063148669e9378e9f9cbf2b6
record_format	dspace
spelling	oai:doaj.org-article:424fecfe063148669e9378e9f9cbf2b62021-11-10T08:53:19ZEffect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats1561-41072251-7170https://doaj.org/article/424fecfe063148669e9378e9f9cbf2b62012-07-01T00:00:00Zhttp://jbums.org/article-1-4153-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Acetaminophen overdose is the most frequent cause of liver injuries. N-acetyl-p-benzoquinone imine (NAPQI) has been proposed as the toxic metabolite of acetaminophen induced by cytochrome P-450. Piperine has antioxidant activities and has been introduced as an inhibitor of CYP3A and P4502E1 activities. This study was done to evaluate pretreatment effect of piperine in acetaminophen induced hepatotoxicity in rat.METHODS: In this study, the rats (weighing 150-250g) were divided into 5 groups: saline, piperine (10 mg/kg), acetaminophen (sequential doses of 500 and 1000 mg/kg within 18 hours), piperine with acetaminophen and silymarin (25 mg/kg) with acetaminophen. Activity of alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase in serum were evaluated and the total antioxidant activity was measured by FRAP method.FINDINGS: Piperine as pretreatment prevented increasing in AST and ALT after acute acetaminophen poisoning (respectively, 1711.25±1108.65 and 496±463.55, compared to acetaminophen, 3956.29±5934.73 and 1914.57±3413.47). Total antioxidant level in acetaminophen group was higher than the saline and piperine groups (respectively, p<0.009 and p<0.003) but no statistical significant differences in results were seen between piperine- acetaminophen and silymarin-acetaminophen groups.CONCLUSION: Piperine may partially prevent increasing level of the enzymes after acetaminophen poisoning. It may be concluded that in presence of piperine the level of antioxidants trends to be decreased. In other word, piperine can decrease the need of high antioxidant capacity to deal NAPQI produced following acetaminophen induced hepatotoxicity.MT KazemiM Pour NasrollahMM Rezaei,GH.A JorsaraeiGH MalijiS KazemiE Zabihi,M Pouramir,AA MoghadamniaBabol University of Medical Sciencesarticleacetaminophenpiperinesilymarinn-acetyl-p-benzoquinone imineaspartate aminotransferasealanine aminotransferaseantioxidantferric reducing ability of plasmaMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 4, Pp 7-14 (2012)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	acetaminophen piperine silymarin n-acetyl-p-benzoquinone imine aspartate aminotransferase alanine aminotransferase antioxidant ferric reducing ability of plasma Medicine R Medicine (General) R5-920
spellingShingle	acetaminophen piperine silymarin n-acetyl-p-benzoquinone imine aspartate aminotransferase alanine aminotransferase antioxidant ferric reducing ability of plasma Medicine R Medicine (General) R5-920 MT Kazemi M Pour Nasrollah MM Rezaei, GH.A Jorsaraei GH Maliji S Kazemi E Zabihi, M Pouramir, AA Moghadamnia Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats
description	BACKGROUND AND OBJECTIVE: Acetaminophen overdose is the most frequent cause of liver injuries. N-acetyl-p-benzoquinone imine (NAPQI) has been proposed as the toxic metabolite of acetaminophen induced by cytochrome P-450. Piperine has antioxidant activities and has been introduced as an inhibitor of CYP3A and P4502E1 activities. This study was done to evaluate pretreatment effect of piperine in acetaminophen induced hepatotoxicity in rat.METHODS: In this study, the rats (weighing 150-250g) were divided into 5 groups: saline, piperine (10 mg/kg), acetaminophen (sequential doses of 500 and 1000 mg/kg within 18 hours), piperine with acetaminophen and silymarin (25 mg/kg) with acetaminophen. Activity of alkaline phosphatase, alanine aminotransferase and aspartate aminotransferase in serum were evaluated and the total antioxidant activity was measured by FRAP method.FINDINGS: Piperine as pretreatment prevented increasing in AST and ALT after acute acetaminophen poisoning (respectively, 1711.25±1108.65 and 496±463.55, compared to acetaminophen, 3956.29±5934.73 and 1914.57±3413.47). Total antioxidant level in acetaminophen group was higher than the saline and piperine groups (respectively, p<0.009 and p<0.003) but no statistical significant differences in results were seen between piperine- acetaminophen and silymarin-acetaminophen groups.CONCLUSION: Piperine may partially prevent increasing level of the enzymes after acetaminophen poisoning. It may be concluded that in presence of piperine the level of antioxidants trends to be decreased. In other word, piperine can decrease the need of high antioxidant capacity to deal NAPQI produced following acetaminophen induced hepatotoxicity.
format	article
author	MT Kazemi M Pour Nasrollah MM Rezaei, GH.A Jorsaraei GH Maliji S Kazemi E Zabihi, M Pouramir, AA Moghadamnia
author_facet	MT Kazemi M Pour Nasrollah MM Rezaei, GH.A Jorsaraei GH Maliji S Kazemi E Zabihi, M Pouramir, AA Moghadamnia
author_sort	MT Kazemi
title	Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats
title_short	Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats
title_full	Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats
title_fullStr	Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats
title_full_unstemmed	Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats
title_sort	effect of piperine pretreatment on biochemical profiles of acetaminophen-induced hepatotoxicity in rats
publisher	Babol University of Medical Sciences
publishDate	2012
url	https://doaj.org/article/424fecfe063148669e9378e9f9cbf2b6
work_keys_str_mv	AT mtkazemi effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT mpournasrollah effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT mmrezaei effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT ghajorsaraei effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT ghmaliji effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT skazemi effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT ezabihi effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT mpouramir effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats AT aamoghadamnia effectofpiperinepretreatmentonbiochemicalprofilesofacetaminopheninducedhepatotoxicityinrats
_version_	1718440366631288832

Effect of Piperine Pretreatment on Biochemical Profiles of Acetaminophen-Induced Hepatotoxicity in Rats

Ejemplares similares