Genome editing in the mushroom-forming basidiomycete Coprinopsis cinerea, optimized by a high-throughput transformation system

Abstract Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay...

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Auteurs principaux: Shigeo S. Sugano, Hiroko Suzuki, Eisuke Shimokita, Hirofumi Chiba, Sumihare Noji, Yuriko Osakabe, Keishi Osakabe
Format: article
Langue:EN
Publié: Nature Portfolio 2017
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Accès en ligne:https://doaj.org/article/42f047196f1f4130a92caee3e7887420
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