IgE Trimers Drive SPE-7 Cytokinergic Activity
Abstract Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells’ receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radi...
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Nature Portfolio
2017
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oai:doaj.org-article:43a4bcae3fcb47bd993a61cc883f32f72021-12-02T12:30:36ZIgE Trimers Drive SPE-7 Cytokinergic Activity10.1038/s41598-017-08212-62045-2322https://doaj.org/article/43a4bcae3fcb47bd993a61cc883f32f72017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08212-6https://doaj.org/toc/2045-2322Abstract Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells’ receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radically anomalous property, termed “cytokinergic”, of inducing basophil degranulation without the intervention of an antigen. We show here that the IgE monomer, freed of protein contaminants, is devoid of this activity, and that the source of the anomaly is a trace impurity, identified as a dissociation-resistant IgE trimer. Possible models for the formation of IgE trimers and the manner in which they cross-link cell surface receptors are suggested herein.Heather J. BaxHolly BowenRebecca L. BeavilRaymond ChungMalcolm WardAnna M. DaviesTihomir S. DodevJames M. McDonnellAndrew J. BeavilBrian J. SuttonHannah J. GouldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Heather J. Bax Holly Bowen Rebecca L. Beavil Raymond Chung Malcolm Ward Anna M. Davies Tihomir S. Dodev James M. McDonnell Andrew J. Beavil Brian J. Sutton Hannah J. Gould IgE Trimers Drive SPE-7 Cytokinergic Activity |
| description |
Abstract Degranulation of mast cells and basophils, with release of agents of the allergic response, ensues when multivalent antigens bind to and cross-link the cells’ receptor-bound IgE antibodies. A widely used commercial monoclonal IgE antibody, SPE-7 IgE from Sigma, was found to possess the radically anomalous property, termed “cytokinergic”, of inducing basophil degranulation without the intervention of an antigen. We show here that the IgE monomer, freed of protein contaminants, is devoid of this activity, and that the source of the anomaly is a trace impurity, identified as a dissociation-resistant IgE trimer. Possible models for the formation of IgE trimers and the manner in which they cross-link cell surface receptors are suggested herein. |
| format |
article |
| author |
Heather J. Bax Holly Bowen Rebecca L. Beavil Raymond Chung Malcolm Ward Anna M. Davies Tihomir S. Dodev James M. McDonnell Andrew J. Beavil Brian J. Sutton Hannah J. Gould |
| author_facet |
Heather J. Bax Holly Bowen Rebecca L. Beavil Raymond Chung Malcolm Ward Anna M. Davies Tihomir S. Dodev James M. McDonnell Andrew J. Beavil Brian J. Sutton Hannah J. Gould |
| author_sort |
Heather J. Bax |
| title |
IgE Trimers Drive SPE-7 Cytokinergic Activity |
| title_short |
IgE Trimers Drive SPE-7 Cytokinergic Activity |
| title_full |
IgE Trimers Drive SPE-7 Cytokinergic Activity |
| title_fullStr |
IgE Trimers Drive SPE-7 Cytokinergic Activity |
| title_full_unstemmed |
IgE Trimers Drive SPE-7 Cytokinergic Activity |
| title_sort |
ige trimers drive spe-7 cytokinergic activity |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/43a4bcae3fcb47bd993a61cc883f32f7 |
| work_keys_str_mv |
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| _version_ |
1718394329401131008 |