Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

Rapid and site-specific deep phosphoproteome profiling by data-independent acquisition without the need for spectral libraries

Localizing phosphorylation sites by data-independent acquisition (DIA)-based proteomics is still challenging. Here, the authors develop algorithms for phosphosite localization and stoichiometry determination, and incorporate them into single-shot DIA-phosphoproteomics workflows.

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Autores principales: Dorte B. Bekker-Jensen, Oliver M. Bernhardt, Alexander Hogrebe, Ana Martinez-Val, Lynn Verbeke, Tejas Gandhi, Christian D. Kelstrup, Lukas Reiter, Jesper V. Olsen
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Science
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Acceso en línea:https://doaj.org/article/448cb8a30d124c708cc21f898c76c600
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https://doaj.org/article/448cb8a30d124c708cc21f898c76c600

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